phospho-Dishevelled 2 (Thr224) Polyclonal Antibody, FITC Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-9060R-FITC-100uL |
| Product Name | phospho-Dishevelled 2 (Thr224) Polyclonal Antibody, FITC Conjugated |
| Applications |
WB
IF(IHC-P)
|
| Specificity | This antibody will recognize DVL3 when phosphorylated at Thr205 |
| Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Dog, Cow, Sheep, Pig, Horse, Chicken |
| Specifications | |
| Conjugation | FITC |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human DVL2 around the phosphorylation site of Thr224 |
| Modification Site | Thr224 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 1856 |
| Swiss Prot | O14641 |
| Subcellular location | Cytoplasm |
| Synonyms | Segment polarity protein dishevelled homolog DVL-2; Dishevelled-2; DSH homolog 2; DVL2 |
| Background | Participates in Wnt signaling by binding to the cytoplasmic C-terminus of frizzled family members and transducing the Wnt signal to down-stream effectors. Promotes internalization and degradation of frizzled proteins upon Wnt signaling. Plays a role both in canonical and non-canonical Wnt signaling. Plays a role in the signal transduction pathways mediated by multiple Wnt genes (By similarity). |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
Frequently asked questions
Which applications is phospho-Dishevelled 2 (Thr224) Polyclonal Antibody, FITC Conjugated (bs-9060R-FITC) validated for, and at what dilution?
bs-9060R-FITC is validated for WB (1:300-5000); IF(IHC-P) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does phospho-Dishevelled 2 (Thr224) Polyclonal Antibody, FITC Conjugated (bs-9060R-FITC) react with?
Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat, Dog, Cow, Sheep, Pig, Horse, Chicken.
How should bs-9060R-FITC be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-Dishevelled 2 (Thr224) Polyclonal Antibody, FITC Conjugated (bs-9060R-FITC)?
KLH conjugated synthetic phosphopeptide derived from human DVL2 around the phosphorylation site of Thr224
What are the excitation and emission wavelengths of the FITC conjugate (bs-9060R-FITC)?
FITC on bs-9060R-FITC: excitation / emission 494nm/518nm.