HMBS Polyclonal Antibody, AbBy Fluor-488 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-9068R-BF488-100uL |
| Product Name | HMBS Polyclonal Antibody, AbBy Fluor-488 Conjugated |
| Applications |
IF
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | AbBy Fluor-488 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human HMBS/PBGD |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 3145 |
| Synonyms | HEM3_HUMAN; HMBS; Hydroxymethylbilane synthase; PBG D; PBG-D; PBGD; Porphobilinogen deaminase; Pre uroporphyrinogen synthase; Pre-uroporphyrinogen synthase; UPS; Uroporphyrinogen I synthase; Uroporphyrinogen I synthetase. |
| Background | PBGD, also designated hydroxymethylbilane synthase, is a cytoplasmic enzyme found in the heme synthesis pathway. PBGD belongs to the HMBS (hydroxymethylbilane synthase) family. Deficiency of PBGD causes errors in pyrrole metabolism, which in turn leads to an inherited autosomal disorder called acute intermittent porphyria (AIP). AIP is characterized by acute attacks of neurological dysfunctions with hypertension, tachycardia, peripheral neurologic disturbances, abdominal pain and excessive amounts of aminolevulinic acid and porphobilinogen in the urine. |
| Application Dilution | |
| IF | WB1:300-5000 |
Frequently asked questions
Which applications is HMBS Polyclonal Antibody, AbBy Fluor-488 Conjugated (bs-9068R-BF488) validated for, and at what dilution?
bs-9068R-BF488 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does HMBS Polyclonal Antibody, AbBy Fluor-488 Conjugated (bs-9068R-BF488) react with?
Tested reactivity: Human, Mouse, Rat.
How should bs-9068R-BF488 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise HMBS Polyclonal Antibody, AbBy Fluor-488 Conjugated (bs-9068R-BF488)?
KLH conjugated synthetic peptide derived from human HMBS/PBGD
What are the excitation and emission wavelengths of the AbBy Fluor-488 conjugate (bs-9068R-BF488)?
AbBy Fluor-488 on bs-9068R-BF488: excitation / emission 499nm/519nm.