HRP2 Polyclonal Antibody, AbBy Fluor-350 Conjugated
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-9072R-BF350 |
| Product Name | HRP2 Polyclonal Antibody, AbBy Fluor-350 Conjugated |
| Applications |
WB
IF(IHC-P)
|
| Predicted Reactivity | Human, Mouse, Rat, Dog, Cow, Sheep, Pig, Chicken |
| Specifications | |
| Conjugation | AbBy Fluor-350 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human HRP2 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Synonyms | HDGF-2; Hdgfrp2; HDGR2_HUMAN; Hepatoma-derived growth factor 2; Hepatoma-derived growth factor; Hepatoma-derived growth factor-related protein 2; heptoma derived growth factor related protein 2; HRP-2. |
| Background | Hepatoma Derived Growth Factor (HDGF) is the original member of a family of polypeptides designated HDGF-related proteins (HRPs). HDGF was initially characterized as a secreted mitogen from the Huh-7 human hepatoma cell line. This nuclear targeted vascular smooth muscle cell mitogen (VSM) is a heparin-binding protein that is highly expressed in tumor cells where it stimulates proliferation. HDGF is also reported to be involved in organ development and lung remodeling after injury by promoting proliferation of lung epithelial cells. During development, HDGF expression is high in the nucleus and cytoplasm of smooth muscle and endothelial cells. The HRP (HDGF related proteins) family contains four proteins, HRP-1, HRP-2, HRP-3 and HRP-4. HRP-1 and HRP-4 are only expressed in testis while HRP-2 is widely expressed in different tissues. HRP-3 can solely be found in the nervous system. Specifically it is strongly expressed in bulbus, olfactorius, piriform cotrex and amygdala complex while HRP-2 in brain is located in the the thalamus, prefrontal and parietal cortex, neurohypophysis, and the cerebellum. In the central nervous system, HRP proteins are play a role in neuron proliferation and cell survival. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
Frequently asked questions
Which applications is HRP2 Polyclonal Antibody, AbBy Fluor-350 Conjugated (bs-9072R-BF350) validated for, and at what dilution?
bs-9072R-BF350 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should bs-9072R-BF350 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise HRP2 Polyclonal Antibody, AbBy Fluor-350 Conjugated (bs-9072R-BF350)?
KLH conjugated synthetic peptide derived from human HRP2
What are the excitation and emission wavelengths of the AbBy Fluor-350 conjugate (bs-9072R-BF350)?
AbBy Fluor-350 on bs-9072R-BF350: excitation / emission 346nm/442nm.