CDK5RAP1 Polyclonal Antibody, RBITC Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-9075R-RBITC-100uL |
| Product Name | CDK5RAP1 Polyclonal Antibody, RBITC Conjugated |
| Applications |
WB
IF(IHC-P)
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | RBITC |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human CDK5RAP1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 51654 |
| Synonyms | CDK5 activator binding protein C42 like; CDK5 regulatory subunit associated protein 1; CDK5RAP1.3; CDK5RAP1.4; CGI 05; HSPC167; C20orf34 antibody C42; CDK5 activator binding protein C42; CK5P1_HUMAN. |
| Background | Cyclin dependent kinase 5 (Cdk5) is a key regulator of cell cycle progression in neuronal differentiation that physically associates with and is activated by the neuron-specific protein p35. CDK5RAP1 (Cdk5 regulatory subunit-associated protein 1), also known as Cdk5 activator-binding protein C42, is a 601 amino acid protein that specifically inhibits Cdk5 activation by p35 through formation of a dimer that inhibits kinase activity. CDK5RAP1 contains one TRAM domain, which is thought to bind tRNA and deliver the RNA-modifying enzymatic domain to its target. There are 4 named isoforms of CDK5RAP1 that are produced as a result of alternative splicing events and are expressed at high levels in heart and skeletal muscle. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
Frequently asked questions
Which applications is CDK5RAP1 Polyclonal Antibody, RBITC Conjugated (bs-9075R-RBITC) validated for, and at what dilution?
bs-9075R-RBITC is validated for WB (1:300-5000); IF(IHC-P) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does CDK5RAP1 Polyclonal Antibody, RBITC Conjugated (bs-9075R-RBITC) react with?
Tested reactivity: Human, Mouse, Rat.
How should bs-9075R-RBITC be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise CDK5RAP1 Polyclonal Antibody, RBITC Conjugated (bs-9075R-RBITC)?
KLH conjugated synthetic peptide derived from human CDK5RAP1
What are the excitation and emission wavelengths of the RBITC conjugate (bs-9075R-RBITC)?
RBITC on bs-9075R-RBITC: excitation / emission 540nm/625nm.