AKIRIN2 Polyclonal Antibody

$Paraformaldehyde-fixed, paraffin embedded mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with AKIRIN2 Polyclonal Antibody, Unconjugated (bs-9082R) at 1:400 overnight at 4\u00b0C, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with AKIRIN2 Polyclonal Antibody, Unconjugated (bs-9082R) at 1:400 overnight at 4\u00b0C, DAB staining.

Applications

WB
IHC-P
IF(IHC-P)

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bs-9082R
Product Name	AKIRIN2 Polyclonal Antibody
Applications	WB, IHC-P, IF(IHC-P)
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human AKIRIN2
Immunogen Range	51-150/203
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Synonyms	AKIR2_HUMAN; AKIRIN 2; Akirin-2; Akirin2; C6orf166; dJ486L4.2; FBI1; FLJ10342; Fourteen three three beta interactant 1.
Background	Required for the innate immune response. Downstream effector of the Toll-like receptor (TLR), TNF and IL-1 beta signaling pathways leading to the production of IL-6. Forms a complex with YWHAB that acts to repress transcription of DUSP1.
Application Dilution
WB	1:300-5000
IHC-P	1:200-400
IF(IHC-P)	1:50-200

Applications

Reactivity

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