FBXO4 Polyclonal Antibody, Cy3 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-9087R-Cy3-100uL |
| Product Name | FBXO4 Polyclonal Antibody, Cy3 Conjugated |
| Applications |
WB
IF
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human FBXO4 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 26272 |
| Synonyms | DKFZp547N213; F box only protein 4; F box protein 4; F box protein Fbx4; FBX4; FBXO 4; FLJ10141. |
| Background | FBXO4 is a substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. It probably recognizes and binds to phosphorylated target proteins. It recognizes TERF1 and promotes its ubiquitination together with UBE2D1. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF | IHC-P1:50-200 |
Frequently asked questions
Which applications is FBXO4 Polyclonal Antibody, Cy3 Conjugated (bs-9087R-Cy3) validated for, and at what dilution?
bs-9087R-Cy3 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does FBXO4 Polyclonal Antibody, Cy3 Conjugated (bs-9087R-Cy3) react with?
Tested reactivity: Human, Mouse, Rat.
How should bs-9087R-Cy3 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise FBXO4 Polyclonal Antibody, Cy3 Conjugated (bs-9087R-Cy3)?
KLH conjugated synthetic peptide derived from human FBXO4
What are the excitation and emission wavelengths of the Cy3 conjugate (bs-9087R-Cy3)?
Cy3 on bs-9087R-Cy3: excitation / emission 512,550nm/570,615nm.