Tankyrase Polyclonal Antibody, Cy5 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-9104R-Cy5 |
| Product Name | Tankyrase Polyclonal Antibody, Cy5 Conjugated |
| Applications |
WB
IF
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Cy5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Tankyrase |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 8658 |
| Swiss Prot | O95271 |
| Synonyms | TIN1; ARTD5; PARPL; TINF1; TNKS1; pART5; PARP5A; PARP-5a; Tankyrase-1; TANK1; ADP-ribosyltransferase diphtheria toxin-like 5; Poly [ADP-ribose] polymerase 5A; TNKS-1; TRF1-interacting ankyrin-related ADP-ribose polymerase; Tankyrase I; TNKS |
| Background | Poly-ADP-ribosyltransferase involved in various processes such as Wnt signaling pathway, telomere length and vesicle trafficking. Acts as an activator of the Wnt signaling pathway by mediating poly-ADP-ribosylation (PARsylation) of AXIN1 and AXIN2, 2 key components of the beta-catenin destruction complex: poly-ADP-ribosylated target proteins are recognized by RNF146, which mediates their ubiquitination and subsequent degradation. Also mediates PARsylation of BLZF1 and CASC3, followed by recruitment of RNF146 and subsequent ubiquitination. Mediates PARsylation of TERF1, thereby contributing to the regulation of telomere length. Involved in centrosome maturation during prometaphase by mediating PARsylation of HEPACAM2/MIKI. May also regulate vesicle trafficking and modulate the subcellular distribution of SLC2A4/GLUT4-vesicles. May be involved in spindle pole assembly through PARsylation of NUMA1. Stimulates 26S proteasome activity. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF | IHC-P1:50-200 |
Frequently asked questions
Which applications is Tankyrase Polyclonal Antibody, Cy5 Conjugated (bs-9104R-Cy5) validated for, and at what dilution?
bs-9104R-Cy5 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Tankyrase Polyclonal Antibody, Cy5 Conjugated (bs-9104R-Cy5) react with?
Tested reactivity: Human, Mouse, Rat.
How should bs-9104R-Cy5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Tankyrase Polyclonal Antibody, Cy5 Conjugated (bs-9104R-Cy5)?
KLH conjugated synthetic peptide derived from human Tankyrase
What are the excitation and emission wavelengths of the Cy5 conjugate (bs-9104R-Cy5)?
Cy5 on bs-9104R-Cy5: excitation / emission 625,650nm/670nm.