POP1 Polyclonal Antibody, Cy7 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-9107R-Cy7 |
| Product Name | POP1 Polyclonal Antibody, Cy7 Conjugated |
| Applications |
WB
IF(IHC-P)
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Cy7 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human ASC2/POP1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 10940 |
| Synonyms | PAAD domain only protein; POP1; PYDC1; Pyrin domain containing 1; Pyrin only protein 1; PYDC1_HUMAN. |
| Background | The death domain (DD) superfamily of proteins share one or more of the following domains: the DD, DED (death-effector domain), CARD (caspase-recruitment domain) and PYD (Pyrin domain). Each of these domains is characterized by a canonical death domain fold, which consists of a bundle of five or six antiparallel -helices. As their names suggest, these domains play prominent roles in programmed cell death. ASC2 (apoptosis-associated speck-like protein containing a CARD 2), also known as Pyrin-only protein 1 or PADD-only protein 1, is an 89 amino acid member of the DD superfamily that contains one Pyrin domain. Localized to the cytoplasm, ASC2 interacts with ASC to modulate NF-B and pro-caspase-1 regulation. ASC2 is predominantly expressed in monocytes, macrophages and granulocytes. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
Frequently asked questions
Which applications is POP1 Polyclonal Antibody, Cy7 Conjugated (bs-9107R-Cy7) validated for, and at what dilution?
bs-9107R-Cy7 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does POP1 Polyclonal Antibody, Cy7 Conjugated (bs-9107R-Cy7) react with?
Tested reactivity: Human, Mouse, Rat.
How should bs-9107R-Cy7 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise POP1 Polyclonal Antibody, Cy7 Conjugated (bs-9107R-Cy7)?
KLH conjugated synthetic peptide derived from human ASC2/POP1
What are the excitation and emission wavelengths of the Cy7 conjugate (bs-9107R-Cy7)?
Cy7 on bs-9107R-Cy7: excitation / emission 743nm/767nm.