ATAD3A Polyclonal Antibody, Cy5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-9108R-Cy5-100uL |
| Product Name | ATAD3A Polyclonal Antibody, Cy5 Conjugated |
| Applications |
WB
IF(IHC-P)
|
| Reactivity | Mouse, Rat |
| Predicted Reactivity | Human, Cow, Sheep, Pig |
| Specifications | |
| Conjugation | Cy5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human ATAD3A |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 55210 |
| Synonyms | AAA-ATPase TOB3; ATAD3B; ATAD3C; Atad3a; ATPase family AAA domain containing protein 3A; ATPase family AAA domain-containing protein 3; ATPase family, AAA domain containing 3A; FLJ10709; FLJ35514; AAA ATPase TOB3; AAA-TOB3; ATPase family AAA domain containing protein 3B; ATPase family, AAA domain containing 3B; ATPase family, AAA domain containing, member 3B; TOB3; ATD3A_HUMAN; ATAD3A/B/C. |
| Background | The AAA ATPase family of molecular chaperones are characterized by a highly conserved AAA motif. Composed of 200-250 residues, the AAA domain contains Walker homology sequences and imparts ATPase activity. Members of the AAA ATPase family act as DNA helicases as well as transcription factors and are thought to be involved in several cellular functions such as cell-cycle regulation, protein proteolysis, organelle biogenesis and vesicle-mediated protein transport. Mitochondrial membrane proteins ATAD3A and ATAD3B contribute to the stabilization of nucleoids which are large mitochondrial DNA (mtDNA)-protein complexes. ATAD3A/B may participate in the transformation pathway and the chemosensitivity of oligodendrogliomas. The genes encoding ATAD3A/B/C maps to human chromosome 1, which houses over 3,000 genes and is the largest human chromosome spanning about 260 million base pairs and making up 8% of the human genome. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
Frequently asked questions
Which applications is ATAD3A Polyclonal Antibody, Cy5 Conjugated (bs-9108R-Cy5) validated for, and at what dilution?
bs-9108R-Cy5 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does ATAD3A Polyclonal Antibody, Cy5 Conjugated (bs-9108R-Cy5) react with?
Tested reactivity: Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Cow, Sheep, Pig.
How should bs-9108R-Cy5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise ATAD3A Polyclonal Antibody, Cy5 Conjugated (bs-9108R-Cy5)?
KLH conjugated synthetic peptide derived from human ATAD3A
What are the excitation and emission wavelengths of the Cy5 conjugate (bs-9108R-Cy5)?
Cy5 on bs-9108R-Cy5: excitation / emission 625,650nm/670nm.