BAGE2 + BAGE3 Polyclonal Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bs-9114R |
| Product Name | BAGE2 + BAGE3 Polyclonal Antibody |
| Applications |
ELISA
IHC-P
IHC-F
IF
|
| Reactivity | Human |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human BAGE2 |
| Immunogen Range | 55-109/109 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Target | |
| Synonyms | B melanoma antigen 2; B melanoma antigen family, member 2; Cancer/testis antigen 2.2; CT2.2; BAGE2_HUMAN. |
| Background | Members of the BAGE gene family encode antigens that are recognized by cytotoxic T lymphocytes and are also known as CT (cancer/testis) antigens. Generated by juxtacentromeric shuffling of the MLL3 gene, the ancestral BAGE gene was expanded by acrocentric exchanges and/or juxtacentromeric movements.Generally, BAGE proteins are silent in all normal tissues with the exception of testis. BAGE2 and BAGE 3 (B melanoma antigen 2 and 3, respectively), also known as Cancer/testis antigen 2.2 and 2.3 (respectively), are 109 amino acid secreted proteins that are expressed in 22% of melanomas, lung and bladder carcinomas, and are also expressed in normal testis tissue. Like the genes encoding MAGE proteins, BAGE genes are most likely silenced by DNA methylation and/or chromatin compaction in normal tissues other than testis. |
| Application Dilution | |
| ELISA | IHC-P1:200-400 |
| IHC-P | IFIHC-P1:50-200 |
| IHC-F | |
| IF | |
Frequently asked questions
Which applications is BAGE2 + BAGE3 Polyclonal Antibody (bs-9114R) validated for, and at what dilution?
bs-9114R is validated for IHC-P (1:200-400); IF(IHC-P) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does BAGE2 + BAGE3 Polyclonal Antibody (bs-9114R) react with?
Tested reactivity: Human.
How should bs-9114R be stored?
Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise BAGE2 + BAGE3 Polyclonal Antibody (bs-9114R)?
KLH conjugated synthetic peptide derived from human BAGE2 Immunogen range: 55-109/109.