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BAGE4 Polyclonal Antibody, APC-Cy7 Conjugated

Applications

  • IF(IHC-P)

Reactivity

  • Human
Overview
Catalog # bs-9116R-APC-Cy7-100uL
Product Name BAGE4 Polyclonal Antibody, APC-Cy7 Conjugated
Applications
IF(IHC-P)
Reactivity Human
Specifications
Conjugation APC-Cy7
Host Rabbit
Source KLH conjugated synthetic peptide derived from human BAGE4
Immunogen Range 18-39/39
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 3624
Synonyms B melanoma antigen 4; B melanoma antigen family, member 4; BAGE4; BAGE4_HUMAN; Cancer/testis antigen 2.4; CT2.4; MGC119851; MGC119852; MGC119853; MLL3P.
Background Members of the BAGE gene family encode antigens that are recognized by cytotoxic T lymphocytes and are also known as CT (cancer/testis) antigens. Generated by juxtacentromeric shuffling of the MLL3 gene, the ancestral BAGE gene was expanded by acrocentric exchanges and/or juxtacentromeric movements. BAGE, also known as B melanoma antigen 1 or cancer/testis antigen 2.1, is a 43 amino acid member of the BAGE family. Generally, BAGE proteins are not expressed in normal tissues, except in testis, but are expressed highly in melanomas, bladder carcinomas, head and neck squamous cell carcinomas, and lung and breast cancer carcinomas. BAGE proteins are not expressed in renal, colorectal and prostatic carcinomas, leukemias and lymphomas.
Application Dilution
IF(IHC-P) 1:50-200

Frequently asked questions

Which applications is BAGE4 Polyclonal Antibody, APC-Cy7 Conjugated (bs-9116R-APC-Cy7) validated for, and at what dilution?

bs-9116R-APC-Cy7 is validated for IF(IHC-P) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does BAGE4 Polyclonal Antibody, APC-Cy7 Conjugated (bs-9116R-APC-Cy7) react with?

Tested reactivity: Human.

How should bs-9116R-APC-Cy7 be stored?

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise BAGE4 Polyclonal Antibody, APC-Cy7 Conjugated (bs-9116R-APC-Cy7)?

KLH conjugated synthetic peptide derived from human BAGE4 Immunogen range: 18-39/39.

What are the excitation and emission wavelengths of the APC-Cy7 conjugate (bs-9116R-APC-Cy7)?

APC-Cy7 on bs-9116R-APC-Cy7: excitation / emission 650nm/780nm.