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ASB7 Polyclonal Antibody, HRP Conjugated

Applications

  • WB
  • IHC-P

Reactivity

  • Rat

Predicted Reactivity

  • Human
  • Mouse
  • Sheep
  • Horse
  • Chicken
Overview
Catalog # bs-9134R-HRP
Product Name ASB7 Polyclonal Antibody, HRP Conjugated
Applications
WB IHC-P
Reactivity Rat
Predicted Reactivity Human, Mouse, Sheep, Horse, Chicken
Specifications
Conjugation HRP
Host Rabbit
Source KLH conjugated synthetic peptide derived from human ASB7
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 140460
Synonyms Ankyrin repeat and SOCS box containing protein 7; ASB 7; ASB-7; ASB7_HUMAN.
Background Members of the suppressor of cytokine signaling (SOCS) family of proteins contain C-terminal regions of homology called the SOCS box, which serves to couple SOCS proteins and their binding partners with the Elongin B/C complex. Several other families of proteins also contain SOCS boxes, but differ from the SOCS proteins in the type of domain they contain upstream of the SOCS box. The largest family of SOCS box-containing proteins is the ankyrin repeat and SOCS box-containing (ASB) protein family. ASB-7 (ankyrin repeat and SOCS box-containing 7) is a 318 amino acid protein that contains one SOCS box domain and seven ANK repeats and belongs to the ASB family. Existing as multiple alternatively spliced isoforms, ASB-7 functions as a substrate-recognition component of an E3 ubiquitin-protein ligase complex and, working in conjunction with other proteins, plays a role in the ubiquitination and subsequent proteasomal degradation of target proteins.
Application Dilution
WB 1:300-5000
IHC-P 1:200-400

Frequently asked questions

Which applications is ASB7 Polyclonal Antibody, HRP Conjugated (bs-9134R-HRP) validated for, and at what dilution?

bs-9134R-HRP is validated for WB (1:300-5000); IHC-P (1:200-400). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does ASB7 Polyclonal Antibody, HRP Conjugated (bs-9134R-HRP) react with?

Tested reactivity: Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Mouse, Sheep, Horse, Chicken.

How should bs-9134R-HRP be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise ASB7 Polyclonal Antibody, HRP Conjugated (bs-9134R-HRP)?

KLH conjugated synthetic peptide derived from human ASB7