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TRIM35 Polyclonal Antibody, Cy5 Conjugated

Applications

  • IF

Reactivity

  • Human
  • Mouse
  • Rat
Overview
Catalog # bs-9149R-Cy5
Product Name TRIM35 Polyclonal Antibody, Cy5 Conjugated
Applications
IF
Reactivity Human, Mouse, Rat
Specifications
Conjugation Cy5
Host Rabbit
Source KLH conjugated synthetic peptide derived from human TRIM35
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 23087
Synonyms Hemopoietic lineage switch protein 5; HLS5; KIAA1098; Macrophage derived apoptosis inducing RBCC protein; MAIR; NC8; Protein MAIR; Protein Nc8; Tripartite mot containing 35; Tripartite mot containing protein 35; TRI35_HUMAN.
Background TRIM35 is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1, a B-box type 2 and a coiled-coil region. TRIM35 may play a role as a tumor suppressor and is implicated in the cell death mechanism. There are two named isoforms.
Application Dilution
IF WB1:300-5000

Frequently asked questions

Which applications is TRIM35 Polyclonal Antibody, Cy5 Conjugated (bs-9149R-Cy5) validated for, and at what dilution?

bs-9149R-Cy5 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does TRIM35 Polyclonal Antibody, Cy5 Conjugated (bs-9149R-Cy5) react with?

Tested reactivity: Human, Mouse, Rat.

How should bs-9149R-Cy5 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise TRIM35 Polyclonal Antibody, Cy5 Conjugated (bs-9149R-Cy5)?

KLH conjugated synthetic peptide derived from human TRIM35

What are the excitation and emission wavelengths of the Cy5 conjugate (bs-9149R-Cy5)?

Cy5 on bs-9149R-Cy5: excitation / emission 625,650nm/670nm.