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RNF216 Polyclonal Antibody

Applications

  • IHC-P
  • IHC-F
  • IF

Reactivity

  • Human
  • Mouse
  • Rat
Overview
Catalog # bs-9154R
Product Name RNF216 Polyclonal Antibody
Applications
IHC-P IHC-F IF
Reactivity Human, Mouse, Rat
Specifications
Conjugation Unconjugated
Host Rabbit
Source KLH conjugated synthetic peptide derived from human TRIAD3
Immunogen Range 301-400/866
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 54476
Synonyms E3 ubiquitin ligase TRIAD3; ring finger protein 216; RNF216; Triad domain-containing protein 3; U7I1; UBCE7IP1; Ubiquitin conjugating enzyme 7 interacting protein 1; UiP83; ZIN; Zinc finger protein inhibiting NF kappa B; RN216_HUMAN.
Background The TRIAD3 gene encodes a cytoplasmic protein which specifically colocalizes and interacts with the serine/threonine protein kinase, receptor-interacting protein (RIP). Zinc finger domains of the encoded protein are required for its interaction with RIP and for inhibition of TNF- and IL1-induced NF-kappa B activation pathways. The encoded protein may also function as an E3 ubiquitin-protein ligase which accepts ubiquitin from E2 ubiquitin-conjugating enzymes and transfers it to substrates. Several alternatively spliced transcript variants have been described for this locus but the full-length natures of only some are known.
Application Dilution
IHC-P 1:200-400
IHC-F IFIHC-P1:50-200
IF

Frequently asked questions

Which applications is RNF216 Polyclonal Antibody (bs-9154R) validated for, and at what dilution?

bs-9154R is validated for IHC-P (1:200-400); IF(IHC-P) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does RNF216 Polyclonal Antibody (bs-9154R) react with?

Tested reactivity: Human, Mouse, Rat.

How should bs-9154R be stored?

Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise RNF216 Polyclonal Antibody (bs-9154R)?

KLH conjugated synthetic peptide derived from human TRIAD3 Immunogen range: 301-400/866.