RNF216 Polyclonal Antibody, Cy7 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-9154R-Cy7 |
| Product Name | RNF216 Polyclonal Antibody, Cy7 Conjugated |
| Applications |
IF
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Cy7 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human TRIAD3 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 54476 |
| Synonyms | E3 ubiquitin ligase TRIAD3; ring finger protein 216; RNF216; Triad domain-containing protein 3; U7I1; UBCE7IP1; Ubiquitin conjugating enzyme 7 interacting protein 1; UiP83; ZIN; Zinc finger protein inhibiting NF kappa B; RN216_HUMAN. |
| Background | The TRIAD3 gene encodes a cytoplasmic protein which specifically colocalizes and interacts with the serine/threonine protein kinase, receptor-interacting protein (RIP). Zinc finger domains of the encoded protein are required for its interaction with RIP and for inhibition of TNF- and IL1-induced NF-kappa B activation pathways. The encoded protein may also function as an E3 ubiquitin-protein ligase which accepts ubiquitin from E2 ubiquitin-conjugating enzymes and transfers it to substrates. Several alternatively spliced transcript variants have been described for this locus but the full-length natures of only some are known. |
| Application Dilution | |
| IF | IHC-P1:50-200 |
Frequently asked questions
Which applications is RNF216 Polyclonal Antibody, Cy7 Conjugated (bs-9154R-Cy7) validated for, and at what dilution?
bs-9154R-Cy7 is validated for IF(IHC-P) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does RNF216 Polyclonal Antibody, Cy7 Conjugated (bs-9154R-Cy7) react with?
Tested reactivity: Human, Mouse, Rat.
How should bs-9154R-Cy7 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise RNF216 Polyclonal Antibody, Cy7 Conjugated (bs-9154R-Cy7)?
KLH conjugated synthetic peptide derived from human TRIAD3
What are the excitation and emission wavelengths of the Cy7 conjugate (bs-9154R-Cy7)?
Cy7 on bs-9154R-Cy7: excitation / emission 743nm/767nm.