RNF14 Polyclonal Antibody, Cy5 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-9159R-Cy5 |
| Product Name | RNF14 Polyclonal Antibody, Cy5 Conjugated |
| Applications |
WB
IF(IHC-P)
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Cy5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human RNF14 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 9604 |
| Synonyms | Androgen receptor associated protein 54; Androgen receptor-associated protein 54; ARA 54; ARA54; E3 ubiquitin protein ligase RNF14; E3 ubiquitin-protein ligase RNF14; HFB 30; HFB30; HRIHFB2038; RING finger protein 14; RNF 14; Rnf14; RNF14_HUMAN; TRIAD 2; TRIAD2; Triad2 protein. |
| Background | Might act as an E3 ubiquitin-protein ligase which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes and then transfers it to substrates, which could be nuclear proteins. Could play a role as a coactivator for androgen- and, to a lesser extent, progesterone-dependent transcription. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
Frequently asked questions
Which applications is RNF14 Polyclonal Antibody, Cy5 Conjugated (bs-9159R-Cy5) validated for, and at what dilution?
bs-9159R-Cy5 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does RNF14 Polyclonal Antibody, Cy5 Conjugated (bs-9159R-Cy5) react with?
Tested reactivity: Human, Mouse, Rat.
How should bs-9159R-Cy5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise RNF14 Polyclonal Antibody, Cy5 Conjugated (bs-9159R-Cy5)?
KLH conjugated synthetic peptide derived from human RNF14
What are the excitation and emission wavelengths of the Cy5 conjugate (bs-9159R-Cy5)?
Cy5 on bs-9159R-Cy5: excitation / emission 625,650nm/670nm.