RNF22/TRIM3 Polyclonal Antibody, RBITC Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-9174R-RBITC-100uL |
| Product Name | RNF22/TRIM3 Polyclonal Antibody, RBITC Conjugated |
| Applications |
WB
IF(IHC-P)
|
| Reactivity | Rat |
| Predicted Reactivity | Human, Mouse, Dog, Cow, Sheep, Pig, Horse |
| Specifications | |
| Conjugation | RBITC |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human TRIM3 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 10612 |
| Synonyms | Tripartite motif-containing protein 3; TRIM3; Tripartite motif-containing protein 3; Brain-expressed RING finger protein; RING finger protein 22; RING finger protein 97; TRIM3; BERP; RNF22; RNF97 |
| Background | Probably involved in vesicular trafficking via its association with the CART complex (PubMed:15772161). The CART complex is necessary for efficient transferrin receptor recycling but not for EGFR degradation (PubMed:15772161). Positively regulates motility of microtubule-dependent motor protein KIF21B (By similarity). |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
Frequently asked questions
Which applications is RNF22/TRIM3 Polyclonal Antibody, RBITC Conjugated (bs-9174R-RBITC) validated for, and at what dilution?
bs-9174R-RBITC is validated for WB (1:300-5000); IF(IHC-P) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does RNF22/TRIM3 Polyclonal Antibody, RBITC Conjugated (bs-9174R-RBITC) react with?
Tested reactivity: Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Mouse, Dog, Cow, Sheep, Pig, Horse.
How should bs-9174R-RBITC be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise RNF22/TRIM3 Polyclonal Antibody, RBITC Conjugated (bs-9174R-RBITC)?
KLH conjugated synthetic peptide derived from human TRIM3
What are the excitation and emission wavelengths of the RBITC conjugate (bs-9174R-RBITC)?
RBITC on bs-9174R-RBITC: excitation / emission 540nm/625nm.