Repetin Polyclonal Antibody, APC-Cy7 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-9181R-APC-Cy7-100uL |
| Product Name | Repetin Polyclonal Antibody, APC-Cy7 Conjugated |
| Applications |
WB
IF(IHC-P)
|
| Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Cow, Pig, Horse |
| Specifications | |
| Conjugation | APC-Cy7 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Repetin |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 126638 |
| Swiss Prot | Q6XPR3 |
| Synonyms | Intermediate filament associated protein; RPTN; RPTN_HUMAN. |
| Background | Repetin is a 784 amino acid secreted protein that belongs to the S100-fused protein family. Repetin is an extracellular epidermal matrix protein that is expressed in the epidermis and at high levels in eccrine sweat glands, the inner sheaths of hair roots and the filiform papilli of the tongue. A multifunctional epidermal matrix protein, repetin is involved in the cornified cell envelope formation. Containing two EF-hand domains, repetin?s recombinant N-terminal domain binds calcium reversibly. Repetin and trichohyalin may compensate for absent loricrin. The repetin gene contains three exons, two introns and spans at least 5.6 kb. The repetin gene is conserved in cow, mouse and rat, and maps to human chromosome 1q21.3. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
Frequently asked questions
Which applications is Repetin Polyclonal Antibody, APC-Cy7 Conjugated (bs-9181R-APC-Cy7) validated for, and at what dilution?
bs-9181R-APC-Cy7 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Repetin Polyclonal Antibody, APC-Cy7 Conjugated (bs-9181R-APC-Cy7) react with?
Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat, Cow, Pig, Horse.
How should bs-9181R-APC-Cy7 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Repetin Polyclonal Antibody, APC-Cy7 Conjugated (bs-9181R-APC-Cy7)?
KLH conjugated synthetic peptide derived from human Repetin
What are the excitation and emission wavelengths of the APC-Cy7 conjugate (bs-9181R-APC-Cy7)?
APC-Cy7 on bs-9181R-APC-Cy7: excitation / emission 650nm/780nm.