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SLFN14 Polyclonal Antibody, AbBy Fluor-680 Conjugated

Applications

  • WB
  • IF(IHC-P)

Reactivity

  • Mouse

Predicted Reactivity

  • Human
  • Rat
  • Dog
  • Cow
  • Sheep
  • Pig
  • Rabbit
Overview
Catalog # bs-9190R-BF680
Product Name SLFN14 Polyclonal Antibody, AbBy Fluor-680 Conjugated
Applications
WB IF(IHC-P)
Reactivity Mouse
Predicted Reactivity Human, Rat, Dog, Cow, Sheep, Pig, Rabbit
Specifications
Conjugation AbBy Fluor-680
Host Rabbit
Source KLH conjugated synthetic peptide derived from human SLFN14
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 342618
Swiss Prot P0C7P3
Synonyms Schlafen family member 14; SLFN14; SLN14_HUMAN.
Background Schlafen family members are preferentially expressed in lymphoid tissues and are differentially regulated during thymocyte maturation. Schlafen proteins function as suppressors of cell growth and are thought to play a role in the maintenance of T cell quiescence. The prototype member of the Schlafen family, Slfn1, is transcriptionally unregulated during thymocyte positive selection, and the induction of Slfn1 induces a G0/G1 arrest, suggesting that Slfn1 participates in the regulation of cell cycle and potentially acts as a determining factor for apoptosis. These proteins all contain a largely conserved core domain within the center of the sequence, and yet they are substantially diversified at the N terminus. Slfn14 (Schlafen family member 14) is a 912 amino acid protein belonging to the Schlafen family. Slfn14 exists as two alternatively spliced isoforms and is encoded by a gene mapping to human chromosome 17q12.
Application Dilution
WB 1:300-5000
IF(IHC-P) 1:50-200

Frequently asked questions

Which applications is SLFN14 Polyclonal Antibody, AbBy Fluor-680 Conjugated (bs-9190R-BF680) validated for, and at what dilution?

bs-9190R-BF680 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does SLFN14 Polyclonal Antibody, AbBy Fluor-680 Conjugated (bs-9190R-BF680) react with?

Tested reactivity: Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Human, Rat, Dog, Cow, Sheep, Pig, Rabbit.

How should bs-9190R-BF680 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise SLFN14 Polyclonal Antibody, AbBy Fluor-680 Conjugated (bs-9190R-BF680)?

KLH conjugated synthetic peptide derived from human SLFN14

What are the excitation and emission wavelengths of the AbBy Fluor-680 conjugate (bs-9190R-BF680)?

AbBy Fluor-680 on bs-9190R-BF680: excitation / emission 679nm/702nm.