IGSF3 Polyclonal Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bs-9196R-100uL |
| Product Name | IGSF3 Polyclonal Antibody |
| Applications |
WB
ELISA
IHC-P
IHC-F
IF
ICC/IF
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human IGSF3 |
| Immunogen Range | 631-730/1194 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. |
| Target | |
| Gene ID | 3321 |
| Swiss Prot | O75054 |
| Subcellular location | Extracellular |
| Synonyms | EWI 3; EWI3; Glu Trp Ile EWI mot containing protein 3; Immunoglobin superfamily member 3; Immunoglobulin superfamily member 3; KIAA0466; MGC117164; V8 antibody; IGSF3_HUMAN. |
| Background | IGSF3, also known as V8 or EWI-3, is a 1,214 amino acid protein. Widely expressed with predominant expression in kidney, placenta and lung, IGSF3 localizes to the membrane and contains an N-terminal signal peptide, eight immunoglobulin (Ig) domains and a transmembrane segment. IGSF3 exhibits strong sequence and structural similarity to CD101 (32% identity), a leukocyte surface protein with seven Ig domains that is believed to play a role in T-cell activation. Despite the structural similarities between IGSF3 and CD101, IGSF3 is not expressed in peripheral blood lymphocytes and does not appear to participate in an immune function. Based on its subcellular localization and the presence of the eight Ig domains, IGSF3 is hypothesized to function as a surface receptor or as a cell adhesion molecule. |
| Application Dilution | |
| WB | 1:300-5000 |
| ELISA | IHC-P1:200-400 |
| IHC-P | IFIHC-P1:50-200 |
| IHC-F | |
| IF | |
| ICC/IF | |
Frequently asked questions
Which applications is IGSF3 Polyclonal Antibody (bs-9196R) validated for, and at what dilution?
bs-9196R is validated for WB (1:300-5000); IHC-P (1:200-400); IF(IHC-P) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does IGSF3 Polyclonal Antibody (bs-9196R) react with?
Tested reactivity: Human, Mouse, Rat.
How should bs-9196R be stored?
Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise IGSF3 Polyclonal Antibody (bs-9196R)?
KLH conjugated synthetic peptide derived from human IGSF3 Immunogen range: 631-730/1194.