Cystatin D Polyclonal Antibody, Cy5 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-9215R-Cy5 |
| Product Name | Cystatin D Polyclonal Antibody, Cy5 Conjugated |
| Applications |
IF(IHC-P)
|
| Reactivity | Human |
| Specifications | |
| Conjugation | Cy5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Cystatin D |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 1473 |
| Synonyms | CST 5; CST5; Cystatin 5; Cystatin5; Cystatin-5; CystatinD; Cystatin D; Cysteine proteinase inhibitor; MGC71922; CYTD_HUMAN. |
| Background | The cystatin superfamily is a well-established family of cysteine protease inhibitors. Cystatins A and B (type 1) are mainly intracellular; cystatins C, D, E/M, F, G, S, SN and SA cystatins are extracellular (type 2); and the kininogens are type 3 cystatins which are intravascular proteins. All true cystatins inhibit cysteine peptidases of the papain family, such as cathepsins, and some also inhibit legumain family enzymes. Cystatin SA, cystatin S and cystatin SN are found primarily in saliva. Cystatin S and SN can also be expressed in tears, urine and seminal fluid. Cystatin C is a related protein which is expressed in brain, thymus, ovary, epididymis and vas deferens. Cystatin D protects against proteinases in the oral cavity, while Cystatin E/M and F moderate the inhibition of cathepsin proteins. The fetuins, part of the cystatin superfamily, are secretable proteins that influence osteogenesis and bone resorption, regulation of the insulin and hepatocyte growth factor receptors and the response to systemic inflammation. High molecular weight kininogen (Kininogen HC) and low molecular weight kininogen (Kininogen LC) have varied roles, though they both inhibit the thrombin- and plasmin-induced aggregation of thrombocytes. |
| Application Dilution | |
| IF(IHC-P) | 1:50-200 |
Frequently asked questions
Which applications is Cystatin D Polyclonal Antibody, Cy5 Conjugated (bs-9215R-Cy5) validated for, and at what dilution?
bs-9215R-Cy5 is validated for IF(IHC-P) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Cystatin D Polyclonal Antibody, Cy5 Conjugated (bs-9215R-Cy5) react with?
Tested reactivity: Human.
How should bs-9215R-Cy5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Cystatin D Polyclonal Antibody, Cy5 Conjugated (bs-9215R-Cy5)?
KLH conjugated synthetic peptide derived from human Cystatin D
What are the excitation and emission wavelengths of the Cy5 conjugate (bs-9215R-Cy5)?
Cy5 on bs-9215R-Cy5: excitation / emission 625,650nm/670nm.