CST8 Polyclonal Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bs-9216R |
| Product Name | CST8 Polyclonal Antibody |
| Applications |
WB
IHC-P
IF(IHC-P)
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human CST8/Cystatin 8 |
| Immunogen Range | 65-142/142 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Target | |
| Gene ID | 10047 |
| Synonyms | CRES; CST 8; Cst8; CST8_HUMAN; cystatin 8 cystatin related epididymal specic; Cystatin 8; Cystatin related epididymal specic; Cystatin related epididymal specic protein; Cystatin related epididymal spermatogenic protein; Cystatin related protein epididymis specic; Cystatin-8; Cystatin-related epididymal spermatogenic protein. |
| Background | The cystatin superfamily is a well-established family of cysteine protease inhibitors. All true cystatins inhibit cysteine peptidases of the papain family, such as cathepsins, while some also inhibit legumain family enzymes. The CRES (cystatin-related epididymal spermatogenic) protein defines a new subgroup in the family 2 cystatins of the cystatin superfamily. CRES proteins lack two of the three consensus sites necessary for the cystatin inhibition of C1 cysteine proteases. They are also preferentially expressed in postmeiotic germ cells, the proximal caput epididymidis, and anterior pituitary gonadotrophs. Therefore, CRES proteins may perform unique and tissue-specific functions in the reproductive and neuroendocrine systems. As a member of the CRES subfamily, Cystatin 8 is a 142 amino acid protein that is expressed in the proximal caput region of the epididymis, where it performs a specialized role during sperm development and maturation. |
| Application Dilution | |
| WB | 1:300-5000 |
| IHC-P | 1:200-400 |
| IF(IHC-P) | 1:50-200 |
Frequently asked questions
Which applications is CST8 Polyclonal Antibody (bs-9216R) validated for, and at what dilution?
bs-9216R is validated for WB (1:300-5000); IHC-P (1:200-400); IF(IHC-P) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does CST8 Polyclonal Antibody (bs-9216R) react with?
Tested reactivity: Human, Mouse, Rat.
How should bs-9216R be stored?
Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise CST8 Polyclonal Antibody (bs-9216R)?
KLH conjugated synthetic peptide derived from human CST8/Cystatin 8 Immunogen range: 65-142/142.