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C1orf149 Polyclonal Antibody, Cy7 Conjugated

Applications

  • WB
  • IF(IHC-P)

Reactivity

  • Mouse

Predicted Reactivity

  • Human
  • Rat
  • Dog
  • Cow
  • Sheep
  • Rabbit
Overview
Catalog # bs-9219R-Cy7
Product Name C1orf149 Polyclonal Antibody, Cy7 Conjugated
Applications
WB IF(IHC-P)
Reactivity Mouse
Predicted Reactivity Human, Rat, Dog, Cow, Sheep, Rabbit
Specifications
Conjugation Cy7
Host Rabbit
Source KLH conjugated synthetic peptide derived from human C1orf149
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 64769
Synonyms CDABP0189; Chromosome 1 open reading frame 149; Eaf6; FLJ11730; Hypothetical protein LOC64769; NY SAR 91; RP3 423B22.2; Sarcoma antigen NY SAR 91; EAF6_HUMAN.
Background MEAF6, also known as Eaf6 or NY-SAR-91, is a 191 amino acid nuclear protein belonging to the EAF6 family. MEAF6 is a component of the NuA4 histone acetyltransferase complex, which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histone H4 and H2A. The gene encoding MEAF6 localizes to chromosome 1 and, due to alternative splicing events, MEAF6 exists in at least three isoforms. Chromosome 1 is the largest human chromosome spanning about 260 million base pairs and making up 8% of the human genome. There are about 3,000 genes on chromosome 1, and considering the great number of genes there are also a large number of diseases associated with chromosome 1 such as Hutchinson-Gilford progeria, Stickler syndrome, Parkinsons, Gaucher disease and Usher syndrome.
Application Dilution
WB 1:300-5000
IF(IHC-P) 1:50-200

Frequently asked questions

Which applications is C1orf149 Polyclonal Antibody, Cy7 Conjugated (bs-9219R-Cy7) validated for, and at what dilution?

bs-9219R-Cy7 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does C1orf149 Polyclonal Antibody, Cy7 Conjugated (bs-9219R-Cy7) react with?

Tested reactivity: Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Human, Rat, Dog, Cow, Sheep, Rabbit.

How should bs-9219R-Cy7 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise C1orf149 Polyclonal Antibody, Cy7 Conjugated (bs-9219R-Cy7)?

KLH conjugated synthetic peptide derived from human C1orf149

What are the excitation and emission wavelengths of the Cy7 conjugate (bs-9219R-Cy7)?

Cy7 on bs-9219R-Cy7: excitation / emission 743nm/767nm.