RNF19B Polyclonal Antibody, RBITC Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-9246R-RBITC-100uL |
| Product Name | RNF19B Polyclonal Antibody, RBITC Conjugated |
| Applications |
WB
IF(IHC-P)
|
| Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Dog, Cow, Sheep, Pig, Chicken, Rabbit |
| Specifications | |
| Conjugation | RBITC |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human RNF19B |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 127544 |
| Synonyms | E3 ubiquitin-protein ligase RNF19B; IBR domain containing 3; IBR domain-containing protein 3; IBRDC3; Natural killer lytic-associated molecule; NKLAM; RING finger protein 19B; RN19B_HUMAN; rnf19b. |
| Background | E3 ubiquitin-protein ligase which accepts ubiquitin from E2 ubiquitin-conjugating enzymes UBE2L3 and UBE2L6 in the form of a thioester and then directly transfers the ubiquitin to targeted substrates, such as UCKL1. Involved in the cytolytic activity of natural killer cells and cytotoxic T-cells. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
Frequently asked questions
Which applications is RNF19B Polyclonal Antibody, RBITC Conjugated (bs-9246R-RBITC) validated for, and at what dilution?
bs-9246R-RBITC is validated for WB (1:300-5000); IF(IHC-P) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does RNF19B Polyclonal Antibody, RBITC Conjugated (bs-9246R-RBITC) react with?
Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat, Dog, Cow, Sheep, Pig, Chicken, Rabbit.
How should bs-9246R-RBITC be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise RNF19B Polyclonal Antibody, RBITC Conjugated (bs-9246R-RBITC)?
KLH conjugated synthetic peptide derived from human RNF19B
What are the excitation and emission wavelengths of the RBITC conjugate (bs-9246R-RBITC)?
RBITC on bs-9246R-RBITC: excitation / emission 540nm/625nm.