RNF125 Polyclonal Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bs-9250r-100uL |
| Product Name | RNF125 Polyclonal Antibody |
| Applications |
WB
ELISA
IHC-P
IHC-F
IF
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human TRAC1/RNF125 |
| Immunogen Range | 1-100/232 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. |
| Target | |
| Gene ID | 9611 |
| Synonyms | T cell RING activation protein 1; E3 ubiquitin protein ligase RNF125; Ring finger protein 125; RNF 125; T cell ring protein identied in activation screen; TRAC 1; TRAC1; RN125_HUMAN. |
| Background | The RING-type zinc finger motif is present in a number of viral and eukaryotic proteins and is made of a conserved cysteine-rich domain that is able to bind two zinc atoms. Proteins that contain this conserved domain are generally involved in the ubiquitination pathway of protein degradation. TRAC-1 (T-cell RING activation protein 1), also known as RNF125 (ring finger protein 125) or E3 ubiquitin-protein ligase RNF125, is a 232 amino acid novel E3 ubiquitin ligase that functions as a positive regulator in the T-cell receptor signaling pathway. Expressed predominantly in lymphoid tissues such as spleen, thymus and bone marrow, TRAC-1 has been found to inhibit pathogen-induced cytokine production and down-regulates HIV replication. |
| Application Dilution | |
| WB | =1:500-2000 |
| ELISA | ELISA=1:5000-10000 |
| IHC-P | IHC-P=1:100-500 |
| IHC-F | IHC-F=1:100-500 |
| IF | IF=1:50-200 |
Frequently asked questions
Which applications is RNF125 Polyclonal Antibody (bs-9250R) validated for, and at what dilution?
bs-9250R is validated for WB (WB=1:500-2000); ELISA (ELISA=1:5000-10000); IHC-P (IHC-P=1:100-500); IHC-F (IHC-F=1:100-500); IF (IF=1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does RNF125 Polyclonal Antibody (bs-9250R) react with?
Tested reactivity: Human, Mouse, Rat.
How should bs-9250R be stored?
Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise RNF125 Polyclonal Antibody (bs-9250R)?
KLH conjugated synthetic peptide derived from human TRAC1/RNF125 Immunogen range: 1-100/232.