RNF128 Polyclonal Antibody, FITC Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-9251R-FITC-100uL |
| Product Name | RNF128 Polyclonal Antibody, FITC Conjugated |
| Applications |
WB
IF
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | FITC |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human RNF128 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 79589 |
| Synonyms | E3 ubiquitin protein ligase RNF128; E3 ubiquitin-protein ligase RNF128; FLJ23516; Gene related to anergy in lymphocytes protein; GRAIL; ring finger protein 128; RN128_HUMAN; RNF128. |
| Background | The protein encoded by this gene is a type I transmembrane protein that localizes to the endocytic pathway. This protein contains a RING zinc-finger motif and has been shown to possess E3 ubiquitin ligase activity. Expression of this gene in retrovirally transduced T cell hybridoma significantly inhibits activation-induced IL2 and IL4 cytokine production. Induced expression of this gene was observed in anergic CD4(+) T cells, which suggested a role in the induction of anergic phenotype. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF | IHC-P1:50-200 |
Frequently asked questions
Which applications is RNF128 Polyclonal Antibody, FITC Conjugated (bs-9251R-FITC) validated for, and at what dilution?
bs-9251R-FITC is validated for WB (1:300-5000); IF(IHC-P) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does RNF128 Polyclonal Antibody, FITC Conjugated (bs-9251R-FITC) react with?
Tested reactivity: Human, Mouse, Rat.
How should bs-9251R-FITC be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise RNF128 Polyclonal Antibody, FITC Conjugated (bs-9251R-FITC)?
KLH conjugated synthetic peptide derived from human RNF128
What are the excitation and emission wavelengths of the FITC conjugate (bs-9251R-FITC)?
FITC on bs-9251R-FITC: excitation / emission 494nm/518nm.