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RNF138 Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded human lung cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with RNF138 Polyclonal Antibody, Unconjugated (bs-9254R) at 1:500 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.
Paraformaldehyde-fixed, paraffin embedded human lung cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with RNF138 Polyclonal Antibody, Unconjugated (bs-9254R) at 1:500 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat testis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with RNF138 Polyclonal Antibody, Unconjugated (bs-9254R) at 1:400 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat testis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with RNF138 Polyclonal Antibody, Unconjugated (bs-9254R) at 1:400 overnight at 4\u00b0C, DAB staining.
Bioss unconjugated antibody vial

Applications

  • WB
  • IHC-P
  • IF(IHC-P)

Reactivity

  • Rat

Predicted Reactivity

  • Human
  • Mouse
  • Dog
  • Cow
  • Sheep
  • Pig
  • Horse
  • Rabbit
Overview
Catalog # bs-9254R
Product Name RNF138 Polyclonal Antibody
Applications
WB IHC-P IF(IHC-P)
Reactivity Rat
Predicted Reactivity Human, Mouse, Dog, Cow, Sheep, Pig, Horse, Rabbit
Specifications
Conjugation Unconjugated
Host Rabbit
Source KLH conjugated synthetic peptide derived from human RNF138
Immunogen Range 51-150/245
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 51444
Swiss Prot Q8WVD3
Subcellular location Nucleus
Synonyms NARF; HSD-4; STRIN; hNARF; E3 ubiquitin-protein ligase RNF138; Nemo-like kinase-associated RING finger protein; NLK-associated RING finger protein; RING finger protein 138; RNF138; HSD4
Background E3 ubiquitin-protein ligase. Together with NLK, involved in the ubiquitination and degradation of TCF/LEF. Also exhibits auto-ubiquitination activity in combination with UBE2K. May act as a negative regulator in the Wnt/beta-catenin-mediated signaling pathway.
Application Dilution
WB 1:300-5000
IHC-P 1:200-400
IF(IHC-P) 1:50-200

Frequently asked questions

Which applications is RNF138 Polyclonal Antibody (bs-9254R) validated for, and at what dilution?

bs-9254R is validated for WB (1:300-5000); IHC-P (1:200-400); IF(IHC-P) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does RNF138 Polyclonal Antibody (bs-9254R) react with?

Tested reactivity: Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Mouse, Dog, Cow, Sheep, Pig, Horse, Rabbit.

How should bs-9254R be stored?

Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise RNF138 Polyclonal Antibody (bs-9254R)?

KLH conjugated synthetic peptide derived from human RNF138 Immunogen range: 51-150/245.

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