RNF138 Polyclonal Antibody, Cy3 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-9254R-Cy3-100uL |
| Product Name | RNF138 Polyclonal Antibody, Cy3 Conjugated |
| Applications |
WB
IF(IHC-P)
|
| Reactivity | Rat |
| Predicted Reactivity | Human, Mouse, Dog, Cow, Sheep, Pig, Horse, Rabbit |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human RNF138 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 51444 |
| Swiss Prot | Q8WVD3 |
| Subcellular location | Nucleus |
| Synonyms | NARF; HSD-4; STRIN; hNARF; E3 ubiquitin-protein ligase RNF138; Nemo-like kinase-associated RING finger protein; NLK-associated RING finger protein; RING finger protein 138; RNF138; HSD4 |
| Background | E3 ubiquitin-protein ligase. Together with NLK, involved in the ubiquitination and degradation of TCF/LEF. Also exhibits auto-ubiquitination activity in combination with UBE2K. May act as a negative regulator in the Wnt/beta-catenin-mediated signaling pathway. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
Frequently asked questions
Which applications is RNF138 Polyclonal Antibody, Cy3 Conjugated (bs-9254R-Cy3) validated for, and at what dilution?
bs-9254R-Cy3 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does RNF138 Polyclonal Antibody, Cy3 Conjugated (bs-9254R-Cy3) react with?
Tested reactivity: Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Mouse, Dog, Cow, Sheep, Pig, Horse, Rabbit.
How should bs-9254R-Cy3 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise RNF138 Polyclonal Antibody, Cy3 Conjugated (bs-9254R-Cy3)?
KLH conjugated synthetic peptide derived from human RNF138
What are the excitation and emission wavelengths of the Cy3 conjugate (bs-9254R-Cy3)?
Cy3 on bs-9254R-Cy3: excitation / emission 512,550nm/570,615nm.