RNF170 Polyclonal Antibody

$Paraformaldehyde-fixed, paraffin embedded rat testis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with RNF170 Polyclonal Antibody (bs-9260R) at 1:400 overnight at 4\u00b0C, followed by SP Kit (Rabbit) and DAB.$

Paraformaldehyde-fixed, paraffin embedded rat testis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with RNF170 Polyclonal Antibody (bs-9260R) at 1:400 overnight at 4\u00b0C, followed by SP Kit (Rabbit) and DAB.

$Paraformaldehyde-fixed, paraffin embedded rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with RNF170 Polyclonal Antibody (bs-9260R) at 1:400 overnight at 4\u00b0C, followed by SP Kit (Rabbit) and DAB.$

Paraformaldehyde-fixed, paraffin embedded rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with RNF170 Polyclonal Antibody (bs-9260R) at 1:400 overnight at 4\u00b0C, followed by SP Kit (Rabbit) and DAB.

Applications

WB
IHC-P
IF(IHC-P)

Reactivity

Predicted Reactivity

Human
Mouse
Dog
Cow
Sheep
Horse
Chicken
Rabbit

Overview
Catalog #	bs-9260R
Product Name	RNF170 Polyclonal Antibody
Applications	WB, IHC-P, IF(IHC-P)
Reactivity	Rat
Predicted Reactivity	Human, Mouse, Dog, Cow, Sheep, Horse, Chicken, Rabbit
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human RNF170
Clonality	Polyclonal
Clone #	#REF!
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	81790
Synonyms	Putative LAG1 interacting protein; Ring finger protein 170; RN170_HUMAN.
Background	The ring finger is a specialized type of zinc finger of 40 to 60 residues that binds two atoms of zinc and mediates protein-protein interactions. There are five known isoforms of RNF170.
Application Dilution
WB	1:300-5000
IHC-P	1:200-400
IF(IHC-P)	1:50-200