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RNF170 Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded rat testis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with RNF170 Polyclonal Antibody (bs-9260R) at 1:400 overnight at 4\u00b0C, followed by SP Kit (Rabbit) and DAB.
Paraformaldehyde-fixed, paraffin embedded rat testis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with RNF170 Polyclonal Antibody (bs-9260R) at 1:400 overnight at 4\u00b0C, followed by SP Kit (Rabbit) and DAB.
Paraformaldehyde-fixed, paraffin embedded rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with RNF170 Polyclonal Antibody (bs-9260R) at 1:400 overnight at 4\u00b0C, followed by SP Kit (Rabbit) and DAB.
Paraformaldehyde-fixed, paraffin embedded rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with RNF170 Polyclonal Antibody (bs-9260R) at 1:400 overnight at 4\u00b0C, followed by SP Kit (Rabbit) and DAB.
Bioss unconjugated antibody vial

Applications

  • WB
  • IHC-P
  • IF(IHC-P)

Reactivity

  • Rat

Predicted Reactivity

  • Human
  • Mouse
  • Dog
  • Cow
  • Sheep
  • Horse
  • Chicken
  • Rabbit
Overview
Catalog # bs-9260R
Product Name RNF170 Polyclonal Antibody
Applications
WB IHC-P IF(IHC-P)
Reactivity Rat
Predicted Reactivity Human, Mouse, Dog, Cow, Sheep, Horse, Chicken, Rabbit
Specifications
Conjugation Unconjugated
Host Rabbit
Source KLH conjugated synthetic peptide derived from human RNF170
Immunogen Range 101-200/258
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 81790
Synonyms Putative LAG1 interacting protein; Ring finger protein 170; RN170_HUMAN.
Background The ring finger is a specialized type of zinc finger of 40 to 60 residues that binds two atoms of zinc and mediates protein-protein interactions. There are five known isoforms of RNF170.
Application Dilution
WB 1:300-5000
IHC-P 1:200-400
IF(IHC-P) 1:50-200

Frequently asked questions

Which applications is RNF170 Polyclonal Antibody (bs-9260R) validated for, and at what dilution?

bs-9260R is validated for WB (1:300-5000); IHC-P (1:200-400); IF(IHC-P) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does RNF170 Polyclonal Antibody (bs-9260R) react with?

Tested reactivity: Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Mouse, Dog, Cow, Sheep, Horse, Chicken, Rabbit.

How should bs-9260R be stored?

Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise RNF170 Polyclonal Antibody (bs-9260R)?

KLH conjugated synthetic peptide derived from human RNF170 Immunogen range: 101-200/258.

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