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RNF170 Polyclonal Antibody, Cy5 Conjugated

Applications

  • WB
  • IF(IHC-P)

Reactivity

  • Rat

Predicted Reactivity

  • Human
  • Mouse
  • Dog
  • Cow
  • Sheep
  • Horse
  • Chicken
  • Rabbit
Overview
Catalog # bs-9260R-Cy5
Product Name RNF170 Polyclonal Antibody, Cy5 Conjugated
Applications
WB IF(IHC-P)
Reactivity Rat
Predicted Reactivity Human, Mouse, Dog, Cow, Sheep, Horse, Chicken, Rabbit
Specifications
Conjugation Cy5
Host Rabbit
Source KLH conjugated synthetic peptide derived from human RNF170
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 81790
Synonyms Putative LAG1 interacting protein; Ring finger protein 170; RN170_HUMAN.
Background The ring finger is a specialized type of zinc finger of 40 to 60 residues that binds two atoms of zinc and mediates protein-protein interactions. There are five known isoforms of RNF170.
Application Dilution
WB 1:300-5000
IF(IHC-P) 1:50-200

Frequently asked questions

Which applications is RNF170 Polyclonal Antibody, Cy5 Conjugated (bs-9260R-Cy5) validated for, and at what dilution?

bs-9260R-Cy5 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does RNF170 Polyclonal Antibody, Cy5 Conjugated (bs-9260R-Cy5) react with?

Tested reactivity: Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Mouse, Dog, Cow, Sheep, Horse, Chicken, Rabbit.

How should bs-9260R-Cy5 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise RNF170 Polyclonal Antibody, Cy5 Conjugated (bs-9260R-Cy5)?

KLH conjugated synthetic peptide derived from human RNF170

What are the excitation and emission wavelengths of the Cy5 conjugate (bs-9260R-Cy5)?

Cy5 on bs-9260R-Cy5: excitation / emission 625,650nm/670nm.