RNF215 Polyclonal Antibody, Cy5 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-9264R-Cy5 |
| Product Name | RNF215 Polyclonal Antibody, Cy5 Conjugated |
| Applications |
WB
IF
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Cy5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human RNF215 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 200312 |
| Swiss Prot | Q9Y6U7 |
| Synonyms | RING finger protein 215; Rnf215; RN215_HUMAN. |
| Background | The RING-type zinc finger motif is present in a number of viral and eukaryotic proteins and is made of a conserved cysteine-rich domain that is able to bind two zinc atoms. Proteins that contain this conserved domain are generally involved in the ubiquitination pathway of protein degradation. RNF215 (ring finger protein 215), is a 377 amino acid multi-pass membrane protein containing one RING-type zinc finger. The gene encoding RNF215 maps to human chromosome 22, which houses over 500 genes and is the second smallest human chromosome. Mutations in several of the genes that map to chromosome 22 are involved in the development of Phelan-McDermid syndrome, Neurofibromatosis type 2, autism and schizophrenia. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF | IHC-P1:50-200 |
Frequently asked questions
Which applications is RNF215 Polyclonal Antibody, Cy5 Conjugated (bs-9264R-Cy5) validated for, and at what dilution?
bs-9264R-Cy5 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does RNF215 Polyclonal Antibody, Cy5 Conjugated (bs-9264R-Cy5) react with?
Tested reactivity: Human, Mouse, Rat.
How should bs-9264R-Cy5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise RNF215 Polyclonal Antibody, Cy5 Conjugated (bs-9264R-Cy5)?
KLH conjugated synthetic peptide derived from human RNF215
What are the excitation and emission wavelengths of the Cy5 conjugate (bs-9264R-Cy5)?
Cy5 on bs-9264R-Cy5: excitation / emission 625,650nm/670nm.