RNF32 Polyclonal Antibody, RBITC Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-9265R-RBITC-100uL |
| Product Name | RNF32 Polyclonal Antibody, RBITC Conjugated |
| Applications |
IF
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | RBITC |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human RNF32 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 140545 |
| Swiss Prot | Q9H0A6 |
| Synonyms | RING finger protein 32; Rnf32; RNF32_HUMAN. |
| Background | The RING-type zinc finger motif is present in a number of viral and eukaryotic proteins and is made of a conserved cysteine-rich domain that is able to bind two zinc atoms. Proteins that contain this conserved domain are generally involved in the ubiquitination pathway of protein degradation. RNF32 (RING finger protein 32), also known as HSD15 or FKSG33, is a 362 amino acid cytoplasmic protein that contains one IQ domain and two RING-type zinc fingers. Highly expressed in testis with lower expression levels in ovary tissue, RNF32 is thought to play a role in spermatogenesis, specifically contributing to the growth and maturation of round spermatids. Six isoforms of RNF32 exist due to alternative splicing events. |
| Application Dilution | |
| IF | IHC-P1:50-200 |
Frequently asked questions
Which applications is RNF32 Polyclonal Antibody, RBITC Conjugated (bs-9265R-RBITC) validated for, and at what dilution?
bs-9265R-RBITC is validated for IF(IHC-P) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does RNF32 Polyclonal Antibody, RBITC Conjugated (bs-9265R-RBITC) react with?
Tested reactivity: Human, Mouse, Rat.
How should bs-9265R-RBITC be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise RNF32 Polyclonal Antibody, RBITC Conjugated (bs-9265R-RBITC)?
KLH conjugated synthetic peptide derived from human RNF32
What are the excitation and emission wavelengths of the RBITC conjugate (bs-9265R-RBITC)?
RBITC on bs-9265R-RBITC: excitation / emission 540nm/625nm.