USP10 Polyclonal Antibody

$Paraformaldehyde-fixed, paraffin embedded Human colon cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with USP10 Polyclonal Antibody, Unconjugated (bs-9267R) at 1:200 overnight at 4\u00b0C, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Human colon cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with USP10 Polyclonal Antibody, Unconjugated (bs-9267R) at 1:200 overnight at 4\u00b0C, DAB staining.

Applications

WB
IHC-P
IF(IHC-P)

Reactivity

Human

Predicted Reactivity

Mouse
Rat
Dog
Pig
Horse
Chicken
Rabbit

Overview
Catalog #	bs-9267R
Product Name	USP10 Polyclonal Antibody
Applications	WB, IHC-P, IF(IHC-P)
Reactivity	Human
Predicted Reactivity	Mouse, Rat, Dog, Pig, Horse, Chicken, Rabbit
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human USP10
Clonality	Polyclonal
Clone #	#REF!
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	9100
Synonyms	Deubiquitinating enzyme 10; Ubiquitin carboxyl terminal hydrolase 10; Ubiquitin carboxyl-terminal hydrolase 10; Ubiquitin specic peptidase 10; Ubiquitin specic processing protease 10; Ubiquitin specic protease 10; Ubiquitin thioesterase 10; Ubiquitin thiolesterase 10; Ubiquitin-specic-processing protease 10; UBP10_HUMAN; UBPO; USP 10; USP10.
Background	Ubiquitin is a highly conserved protein that is covalently linked to other proteins to regulate their function and degradation. This gene encodes a member of the ubiquitin-specific protease family of cysteine proteases. The enzyme specifically cleaves ubiquitin from ubiquitin-conjugated protein substrates. The protein is found in the nucleus and cytoplasm. It functions as a co-factor of the DNA-bound androgen receptor complex, and is inhibited by a protein in the Ras-GTPase pathway. The human genome contains several pseudogenes similar to this gene. Several transcript variants, some protein-coding and others not protein-coding, have been found for this gene. [provided by RefSeq, Jan 2013].
Application Dilution
WB	1:300-5000
IHC-P	1:200-400
IF(IHC-P)	1:50-200