PACRG Polyclonal Antibody, Biotin Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-9289R-Biotin-100uL |
| Product Name | PACRG Polyclonal Antibody, Biotin Conjugated |
| Applications |
WB
IHC-P
|
| Reactivity | Rat |
| Predicted Reactivity | Human, Mouse, Dog, Sheep, Horse, Rabbit, Zebrafish |
| Specifications | |
| Conjugation | Biotin |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human PACRG |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C for 12 months. |
| Target | |
| Gene ID | 135138 |
| Synonyms | GLUP; Molecular chaperone/chaperonin binding protein; PARK 2 co regulated; PARK 2 coregulated; PARK2 co regulated; PARK2 coregulated; PARK2CRG; Parkin co regulated gene protein; Parkin Co-regulated Gene; Parkin coregulated gene protein; RP3 495O10.2; PACRG_HUMAN. |
| Background | The deduced 257 amino acid protein PACRG (Parkin co-regulated gene) shows potential links to the ubiquitin/proteasome system. PACRG and Parkin are attached in a head-to-head arrangement on opposite DNA strands and share a common 5' flanking promoter region. The PACRG gene maps to chromosome 6q26; Northern blot analysis detects PACRG expression in all tissues examined except placenta. Using a positional cloning strategy in 197 Vietnamese leprosy simplex families (i.e. families with two unaffected parents and one affected child), significant connections between leprosy and 17 markers in the 5' regulatory region that PARK2 and PACRG share were observed. Possession of two or more of the 17 risk alleles is highly predictive of leprosy.PACRG is a gene located very close to parkin, in reverse orientation on the chromosome. It is thought to be co-transcribed with parkin by a bi-directional promoter between the two genes. |
| Application Dilution | |
| WB | 1:300-5000 |
| IHC-P | 1:200-400 |
Frequently asked questions
Which applications is PACRG Polyclonal Antibody, Biotin Conjugated (bs-9289R-Biotin) validated for, and at what dilution?
bs-9289R-Biotin is validated for WB (1:300-5000); IHC-P (1:200-400). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does PACRG Polyclonal Antibody, Biotin Conjugated (bs-9289R-Biotin) react with?
Tested reactivity: Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Mouse, Dog, Sheep, Horse, Rabbit, Zebrafish.
How should bs-9289R-Biotin be stored?
Store at -20C for 12 months. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise PACRG Polyclonal Antibody, Biotin Conjugated (bs-9289R-Biotin)?
KLH conjugated synthetic peptide derived from human PACRG