BTBD1 Polyclonal Antibody

$Paraformaldehyde-fixed, paraffin embedded rat testi); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with BTBD) Polyclonal Antibody, Unconjugated (bs-9294R) at 1:500 overnight at 4\u00b0C, followed by a conjugated secondary for 20 minutes and DAB staining.$

Paraformaldehyde-fixed, paraffin embedded rat testi); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with BTBD) Polyclonal Antibody, Unconjugated (bs-9294R) at 1:500 overnight at 4\u00b0C, followed by a conjugated secondary for 20 minutes and DAB staining.

Mouse cerebrum lysates probed with BTBD1 Polyclonal Antibody, Unconjugated (bs-9294R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
IHC-P
IF(IHC-P)

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bs-9294R
Product Name	BTBD1 Polyclonal Antibody
Applications	WB, IHC-P, IF(IHC-P)
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human BTBD1
Immunogen Range	101-200/482
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	53339
Synonyms	BTB POZ domain containing 1; BTB domain containing 1; BTB/POZ domain containing protein 1; BTBD 1; BTBD1; C15orf1; HCV NS5A transactivated protein 8; Hepatitis C virus NS5A transactivated protein 8; NS5ATP8; BTBD1_HUMAN.
Background	BTBD1 contains a proline-rich region and a BTB/POZ domain which are typically involved in protein-protein interactions. The C-terminus of BTBD1 binds topoisomerase I and BTBD1 also interacts with TRIM5 isoform Delta. BTBD1 localizes to cytoplasmic bodies and is essential for myogenic differentiation.
Application Dilution
WB	1:300-5000
IHC-P	1:200-400
IF(IHC-P)	1:50-200

Applications

Reactivity

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