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NNP1 Polyclonal Antibody, Cy7 Conjugated

Applications

  • WB
  • IF(IHC-P)

Reactivity

  • Human
  • Mouse
  • Rat
Overview
Catalog # bs-9300R-Cy7
Product Name NNP1 Polyclonal Antibody, Cy7 Conjugated
Applications
WB IF(IHC-P)
Reactivity Human, Mouse, Rat
Specifications
Conjugation Cy7
Host Rabbit
Source KLH conjugated synthetic peptide derived from human NNP1/RRP1 like protein
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 8568
Synonyms NNP 1; NNP-1; NNP1; Nnp1 homolog nucleolar protein; Novel nuclear protein 1; Nucleolar protein Nop52; Protein NNP 1; Protein NNP1; Ribosomal RNA processing 1 homolog; Ribosomal RNA processing protein 1 homolog A; rrp1; RRP1 like protein; RRP1-like protein; RRP1_HUMAN; RRP1A.
Background Novel nuclear protein 1 (NNP-1), also known as RRP1-like protein or Nucleolar protein Nop52, is a 461 amino acid protein belonging to the RRP1 family. Localized to the nucleolus, NNP-1 has simian virus 40-type and bipartite nuclear localization signals and four coiled-coil domains within its C-terminal region. NNP-1 has been found to play an important role in the generation of 28S rRNA in the late processing steps of ribosome biogenesis. At the end of mitosis, nucleolar proteins assemble in a sequential order during the rebuilding of the nucleolus. NNP-1 assembles after Fibrillarin and C23, and simultaneously with B23 and POP1 in the prenucleolar body pathway.
Application Dilution
WB 1:300-5000
IF(IHC-P) 1:50-200

Frequently asked questions

Which applications is NNP1 Polyclonal Antibody, Cy7 Conjugated (bs-9300R-Cy7) validated for, and at what dilution?

bs-9300R-Cy7 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does NNP1 Polyclonal Antibody, Cy7 Conjugated (bs-9300R-Cy7) react with?

Tested reactivity: Human, Mouse, Rat.

How should bs-9300R-Cy7 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise NNP1 Polyclonal Antibody, Cy7 Conjugated (bs-9300R-Cy7)?

KLH conjugated synthetic peptide derived from human NNP1/RRP1 like protein

What are the excitation and emission wavelengths of the Cy7 conjugate (bs-9300R-Cy7)?

Cy7 on bs-9300R-Cy7: excitation / emission 743nm/767nm.