SASS6 Polyclonal Antibody, Biotin Conjugated
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-9301R-Biotin-100uL |
| Product Name | SASS6 Polyclonal Antibody, Biotin Conjugated |
| Applications |
WB
IHC-P
|
| Predicted Reactivity | Human, Mouse, Rat, Dog, Cow, Sheep, Horse |
| Specifications | |
| Conjugation | Biotin |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human SASS6 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C for 12 months. |
| Target | |
| Gene ID | 163786 |
| Synonyms | HsSAS 6; HsSAS6; MGC119440; SAS 6; SAS6; SASS 6; Spindle assembly 6 homolog C. elegans; Spindle assembly 6 homolog; Spindle assembly abnormal protein 6 homolog; PJA2_HUMAN. |
| Background | SAS-6 (spindle assembly abnormal protein 6 homolog, HsSAS-6) is a 657 amino acid protein encoded by the human gene SAS6. SAS-6 is a component of the centrosome that contains one PISA (present in SAS-6) domain. LK4, SAS-6, CPAP and other centriole related proteins are required at different stages of procentriole formation and were associated with different centriolar structures. SAS-6 associates only transiently with nascent procentrioles, whereas CEP135 and CPAP form a core structure within the proximal lumen of both parental and nascent centrioles. SAS-6 is necessary for procentriole formation in human cell lines and is localized asymmetrically next to the centriole at the onset of procentriole formation. SAS-6 levels oscillate during the cell cycle; it is degraded in mitosis starting at anaphase, and it accumulates again at the end of the following G1 phase. The anaphase-promoting complex targets SAS-6 for degradation by the 26S Proteasome, and a KEN box in the C-terminus of SAS-6 is necessary for its degradation. Increased SAS-6 levels promoted the formation of multiple procentrioles forming next to a single centriole. |
| Application Dilution | |
| WB | 1:300-5000 |
| IHC-P | 1:200-400 |
Frequently asked questions
Which applications is SASS6 Polyclonal Antibody, Biotin Conjugated (bs-9301R-Biotin) validated for, and at what dilution?
bs-9301R-Biotin is validated for WB (1:300-5000); IHC-P (1:200-400). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should bs-9301R-Biotin be stored?
Store at -20C for 12 months. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise SASS6 Polyclonal Antibody, Biotin Conjugated (bs-9301R-Biotin)?
KLH conjugated synthetic peptide derived from human SASS6