SPO11 Polyclonal Antibody, Cy3 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-9302R-Cy3 |
| Product Name | SPO11 Polyclonal Antibody, Cy3 Conjugated |
| Applications |
WB
IF(IHC-P)
|
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Dog, Pig, Horse |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human SPO11 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 23626 |
| Synonyms | Meiotic recombination protein SPO 11; Meiotic recombination protein SPO11; MGC39953; SPO 11; SPO11 meiotic protein covalently bound to DSB homolog; SPO11 meiotic protein covalently bound to DSB like; SPO11_HUMAN. |
| Background | Spo11 is a type II topoisomerase that is thought to generate the chromosome breaks that initiate meiotic recombination. The Spo11 protein initiates meiotic recombination by generating DNA double-strand breaks (DSBs) and is required for meiotic synapsis in S. cerevisiae. The DSBs are located mostly in promoter regions, where the chromatin is in an open configuration, and cluster in domains along the chromosome. Expression of the Spo11 is detected mainly in the testis, in agreement with its predicted function in the initiation of meiotic recombination. Disruption of Spo11 leads to severe gonadal abnormalities from defective meiosis and results in infertility. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
Frequently asked questions
Which applications is SPO11 Polyclonal Antibody, Cy3 Conjugated (bs-9302R-Cy3) validated for, and at what dilution?
bs-9302R-Cy3 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does SPO11 Polyclonal Antibody, Cy3 Conjugated (bs-9302R-Cy3) react with?
Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Dog, Pig, Horse.
How should bs-9302R-Cy3 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise SPO11 Polyclonal Antibody, Cy3 Conjugated (bs-9302R-Cy3)?
KLH conjugated synthetic peptide derived from human SPO11
What are the excitation and emission wavelengths of the Cy3 conjugate (bs-9302R-Cy3)?
Cy3 on bs-9302R-Cy3: excitation / emission 512,550nm/570,615nm.