MAP-9 Polyclonal Antibody, Cy5 Conjugated
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-9313R-Cy5-100uL |
| Product Name | MAP-9 Polyclonal Antibody, Cy5 Conjugated |
| Applications |
WB
IF(IHC-P)
|
| Predicted Reactivity | Human, Mouse, Rat, Dog, Cow, Sheep, Pig, Horse, Rabbit |
| Specifications | |
| Conjugation | Cy5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human MAP-9 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 79884 |
| Synonyms | ASAP; Aster associated protein; MAP9; MAP 9; microtubule associated protein 9; MAP9_HUMAN. |
| Background | Microtubules, the primary component of the cytoskeletal network, interact with proteins called microtubule-associated proteins (MAPs).MAP9 is a microtubule-associated protein required for spindle function, mitotic progression, and cytokinesis.The microtubule-associated proteins can be divided into two groups, structural and dynamic. The MAP proteins function to stimulate tubulin assembly, enhance microtubule stability, influence the spatial distribution of microtubules within cells and utilize microtubule polarity to translocate cellular components. MAP-9 (microtubule-associated protein 9), also known as ASAP, is a 647 amino acid cytoplasmic protein that is constitutively expressed during the cell cycle. MAP-9 localizes to microtubules in interphase, associates with the mitotic spindle during mitosis and localizes to the central body during cytokinesis. Involved in organization of the bipolar mitotic spindle, MAP-9 is required for bipolar spindle assembly, mitosis progression and cytokinesis. MAP-9 may be involved in stabilizing interphase microtubules. Two isoforms of MAP-9 are produced due to alternative splicing events. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
Frequently asked questions
Which applications is MAP-9 Polyclonal Antibody, Cy5 Conjugated (bs-9313R-Cy5) validated for, and at what dilution?
bs-9313R-Cy5 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should bs-9313R-Cy5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise MAP-9 Polyclonal Antibody, Cy5 Conjugated (bs-9313R-Cy5)?
KLH conjugated synthetic peptide derived from human MAP-9
What are the excitation and emission wavelengths of the Cy5 conjugate (bs-9313R-Cy5)?
Cy5 on bs-9313R-Cy5: excitation / emission 625,650nm/670nm.