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NEK9 Polyclonal Antibody, Cy7 Conjugated

Applications

  • WB
  • IF(IHC-P)

Reactivity

  • Mouse

Predicted Reactivity

  • Human
  • Rat
  • Dog
  • Cow
  • Sheep
  • Pig
  • Horse
  • Rabbit
Overview
Catalog # bs-9317R-Cy7-100uL
Product Name NEK9 Polyclonal Antibody, Cy7 Conjugated
Applications
WB IF(IHC-P)
Reactivity Mouse
Predicted Reactivity Human, Rat, Dog, Cow, Sheep, Pig, Horse, Rabbit
Specifications
Conjugation Cy7
Host Rabbit
Source KLH conjugated synthetic peptide derived from human NEK9
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 91754
Synonyms Nek 8; NEK 9; Nek8; nek9; Nek-8; nek-9; NEK9_HUMAN; NERCC 1; Nercc 1 kinase; NERCC; NERCC1; Nercc1 kinase; Never in mitosis A related kinase 9; Never in mitosis A-related kinase 9; NIMA never in mitosis gene a related kinase 9; NIMA related kinase 8; NIMA related kinase 9; NIMA related kinase Nek 8; NIMA related kinase Nek 9; NIMA related kinase Nek8; NIMA related kinase Nek9; NimA related protein kinase 9; NIMA-related kinase 8; NimA-related protein kinase 9; Serine/threonine protein kinase Nek 9; Serine/threonine protein kinase Nek9; Serine/threonine-protein kinase Nek9.
Background Pleiotropic regulator of mitotic progression, participating in the control of spindle dynamics and chromosome separation. Phosphorylates different histones, myelin basic protein, beta-casein, and BICD2. Phosphorylates histone H3 on serine and threonine residues and beta-casein on serine residues. Important for G1/S transition and S phase progression.
Application Dilution
WB 1:300-5000
IF(IHC-P) 1:50-200

Frequently asked questions

Which applications is NEK9 Polyclonal Antibody, Cy7 Conjugated (bs-9317R-Cy7) validated for, and at what dilution?

bs-9317R-Cy7 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does NEK9 Polyclonal Antibody, Cy7 Conjugated (bs-9317R-Cy7) react with?

Tested reactivity: Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Human, Rat, Dog, Cow, Sheep, Pig, Horse, Rabbit.

How should bs-9317R-Cy7 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise NEK9 Polyclonal Antibody, Cy7 Conjugated (bs-9317R-Cy7)?

KLH conjugated synthetic peptide derived from human NEK9

What are the excitation and emission wavelengths of the Cy7 conjugate (bs-9317R-Cy7)?

Cy7 on bs-9317R-Cy7: excitation / emission 743nm/767nm.