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ASZ1 Polyclonal Antibody, AbBy Fluor-750 Conjugated

Applications

  • WB
  • IF(IHC-P)

Reactivity

  • Mouse

Predicted Reactivity

  • Human
  • Rat
  • Horse
Overview
Catalog # bs-9325R-BF750-100uL
Product Name ASZ1 Polyclonal Antibody, AbBy Fluor-750 Conjugated
Applications
WB IF(IHC-P)
Reactivity Mouse
Predicted Reactivity Human, Rat, Horse
Specifications
Conjugation AbBy Fluor-750
Host Rabbit
Source KLH conjugated synthetic peptide derived from human ASZ1
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 136991
Swiss Prot Q8WWH4
Subcellular location Cytoplasm
Synonyms ALP1; GASZ; Orf3; ANKL1; C7orf7; CT1.19; Ankyrin repeat, SAM and basic leucine zipper domain-containing protein 1; Ankyrin-like protein 1; Germ cell-specific ankyrin, SAM and basic leucine zipper domain-containing protein; ASZ1
Background Plays a central role during spermatogenesis by repressing transposable elements and preventing their mobilization, which is essential for the germline integrity. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and governs the methylation and subsequent repression of transposons. Its association with pi-bodies suggests a participation in the primary piRNAs metabolic process. Required prior to the pachytene stage to facilitate the production of multiple types of piRNAs, including those associated with repeats involved in the regulation of retrotransposons. May act by mediating protein-protein interactions during germ cell maturation (By similarity).
Application Dilution
WB 1:300-5000
IF(IHC-P) 1:50-200

Frequently asked questions

Which applications is ASZ1 Polyclonal Antibody, AbBy Fluor-750 Conjugated (bs-9325R-BF750) validated for, and at what dilution?

bs-9325R-BF750 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does ASZ1 Polyclonal Antibody, AbBy Fluor-750 Conjugated (bs-9325R-BF750) react with?

Tested reactivity: Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Human, Rat, Horse.

How should bs-9325R-BF750 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise ASZ1 Polyclonal Antibody, AbBy Fluor-750 Conjugated (bs-9325R-BF750)?

KLH conjugated synthetic peptide derived from human ASZ1

What are the excitation and emission wavelengths of the AbBy Fluor-750 conjugate (bs-9325R-BF750)?

AbBy Fluor-750 on bs-9325R-BF750: excitation / emission 749nm/775nm.