NHLRC2 Polyclonal Antibody, Cy3 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-9332R-Cy3 |
| Product Name | NHLRC2 Polyclonal Antibody, Cy3 Conjugated |
| Applications |
IF
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human NHLRC2 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 374354 |
| Synonyms | DKFZp779F115; FLJ20147; FLJ25621; MGC45492; NHL repeat containing 2; Novel NHL repeat domain containing protein; NHLC2_HUMAN. |
| Background | NHLRC2 (NHL repeat containing 2) is a 726 amino acid protein that contains six NHL repeats and one thioredoxin domain. Existing as two alternatively spliced isoforms, NHLRC2 is encoded by a gene that maps to human chromosome 10q25.3. Spanning nearly 135 million base pairs, chromosome 10 makes up approximately 4.5% of total DNA in cells and encodes nearly 1,200 genes. Several protein-coding genes, including those that encode for chemokines, cadherins, excision repair proteins, early growth response factors (Egrs) and fibroblast growth receptors (FGFRs), are located on chromosome 10. Defects in some of the genes that map to chromosome 10 are associated with Charcot-Marie Tooth disease, Jackson-Weiss syndrome, Usher syndrome, nonsyndromatic deafness, Wolman?s syndrome, Cowden syndrome, multiple endocrine neoplasia type 2 and porphyria. |
| Application Dilution | |
| IF | WB1:300-5000 |
Frequently asked questions
Which applications is NHLRC2 Polyclonal Antibody, Cy3 Conjugated (bs-9332R-Cy3) validated for, and at what dilution?
bs-9332R-Cy3 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does NHLRC2 Polyclonal Antibody, Cy3 Conjugated (bs-9332R-Cy3) react with?
Tested reactivity: Human, Mouse, Rat.
How should bs-9332R-Cy3 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise NHLRC2 Polyclonal Antibody, Cy3 Conjugated (bs-9332R-Cy3)?
KLH conjugated synthetic peptide derived from human NHLRC2
What are the excitation and emission wavelengths of the Cy3 conjugate (bs-9332R-Cy3)?
Cy3 on bs-9332R-Cy3: excitation / emission 512,550nm/570,615nm.