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NHLRC3 Polyclonal Antibody, Cy3 Conjugated

Applications

  • WB
  • IF

Reactivity

  • Human
  • Mouse
  • Rat
Overview
Catalog # bs-9333R-Cy3
Product Name NHLRC3 Polyclonal Antibody, Cy3 Conjugated
Applications
WB IF
Reactivity Human, Mouse, Rat
Specifications
Conjugation Cy3
Host Rabbit
Source KLH conjugated synthetic peptide derived from human NHLRC3
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Synonyms DKFZp313M1221; DKFZp686E1140; NHL repeat containing 3; NHL repeat containing protein 3; NHLRC 3; NHLC3_HUMAN.
Background NHLRC3 contains 4 NHL repeats. The function of the NHLRC3 protein remains unknown. Key tumor suppressor genes on chromosome 13 include the breast cancer susceptibility gene, BRCA2, and the RB1 (retinoblastoma) gene. RB1 encodes a crucial tumor suppressor protein which, when defective, leads to malignant growth in the retina and has been implicated in a variety of other cancers. The gene SLITRK1, which is associated with Tourette syndrome, is on chromosome 13. As with most chromosomes, polysomy of part or all of chromosome 13 is deleterious to development and decreases the odds of survival. Trisomy 13, also known as Patau syndrome, is quite deadly and the few who survive past one year suffer from permanent neurologic defects, difficulty eating and vulnerability to serious respiratory infections.
Application Dilution
WB 1:300-5000
IF IHC-P1:50-200

Frequently asked questions

Which applications is NHLRC3 Polyclonal Antibody, Cy3 Conjugated (bs-9333R-Cy3) validated for, and at what dilution?

bs-9333R-Cy3 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does NHLRC3 Polyclonal Antibody, Cy3 Conjugated (bs-9333R-Cy3) react with?

Tested reactivity: Human, Mouse, Rat.

How should bs-9333R-Cy3 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise NHLRC3 Polyclonal Antibody, Cy3 Conjugated (bs-9333R-Cy3)?

KLH conjugated synthetic peptide derived from human NHLRC3

What are the excitation and emission wavelengths of the Cy3 conjugate (bs-9333R-Cy3)?

Cy3 on bs-9333R-Cy3: excitation / emission 512,550nm/570,615nm.