MARCH3 Polyclonal Antibody, FITC Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-9337R-FITC |
| Product Name | MARCH3 Polyclonal Antibody, FITC Conjugated |
| Applications |
WB
IF(IHC-P)
|
| Reactivity | Mouse |
| Predicted Reactivity | Human, Rat, Cow, Sheep, Pig, Horse, Chicken, Rabbit |
| Specifications | |
| Conjugation | FITC |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human MARCH3 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 115123 |
| Synonyms | E3 ubiquitin-protein ligase MARCH3; FLJ20096; MARCH-III; March3; MARH3_HUMAN; Membrane associated ring finger C3HC4 3; Membrane-associated RING finger protein 3; Membrane-associated RING-CH protein III; RING finger protein 173; RNF173. |
| Background | Ubiquitination is an important mechanism through which three classes of enzymes act in concert to target short-lived or abnormal proteins for destruction. The three classes of enzymes involved in ubiquitination are the ubiquitin-activating enzymes (E1s), the ubiquitin-conjugating enzymes (E2s) and the ubiquitin-protein ligases (E3s). MARCH3 (membrane-associated ring finger (C3HC4) 3), also known as RNF173, is a 253 amino acid multi-pass membrane protein that localizes to cytoplasmic vesicles and early endosomes and contains one RING-CH-type zinc finger. Involved in the pathway of protein modification, MARCH3 functions as an E3 ubiquitin-protein ligase that accepts a ubiquitin residue from an E2 ubiquitin-conjugating enzyme and is thought to be involved in endosomal trafficking events. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
Frequently asked questions
Which applications is MARCH3 Polyclonal Antibody, FITC Conjugated (bs-9337R-FITC) validated for, and at what dilution?
bs-9337R-FITC is validated for WB (1:300-5000); IF(IHC-P) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does MARCH3 Polyclonal Antibody, FITC Conjugated (bs-9337R-FITC) react with?
Tested reactivity: Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Human, Rat, Cow, Sheep, Pig, Horse, Chicken, Rabbit.
How should bs-9337R-FITC be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise MARCH3 Polyclonal Antibody, FITC Conjugated (bs-9337R-FITC)?
KLH conjugated synthetic peptide derived from human MARCH3
What are the excitation and emission wavelengths of the FITC conjugate (bs-9337R-FITC)?
FITC on bs-9337R-FITC: excitation / emission 494nm/518nm.