MARCH4 Polyclonal Antibody, Cy3 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-9338R-Cy3 |
| Product Name | MARCH4 Polyclonal Antibody, Cy3 Conjugated |
| Applications |
WB
IF(IHC-P)
|
| Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Dog, Cow, Sheep, Pig, Horse, Chicken, Rabbit |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human MARCH4 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 57574 |
| Synonyms | E3 ubiquitin protein ligase MARCH4; RNF174; KIAA1399; MARCH 4; MARCH IV; Membrane associated RING CH protein IV; Membrane associated ring finger C3HC4 4; Membrane associated RING finger protein 4; mKIAA1399; RING finger protein 174; MARH4_HUMAN. |
| Background | MARCH4, an E3 ubiquitin protein ligase, may mediate ubiquitination of MHC I and CD4, and promote their subsequent endocytosis and sorting to lysosomes via multivesicular bodies. E3 ubiquitin protein ligases accept ubiquitin from an E2 ubiquitin conjugating enzyme in the form of a thioester and then directly transfer the ubiquitin to targeted substrates. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
Frequently asked questions
Which applications is MARCH4 Polyclonal Antibody, Cy3 Conjugated (bs-9338R-Cy3) validated for, and at what dilution?
bs-9338R-Cy3 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does MARCH4 Polyclonal Antibody, Cy3 Conjugated (bs-9338R-Cy3) react with?
Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat, Dog, Cow, Sheep, Pig, Horse, Chicken, Rabbit.
How should bs-9338R-Cy3 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise MARCH4 Polyclonal Antibody, Cy3 Conjugated (bs-9338R-Cy3)?
KLH conjugated synthetic peptide derived from human MARCH4
What are the excitation and emission wavelengths of the Cy3 conjugate (bs-9338R-Cy3)?
Cy3 on bs-9338R-Cy3: excitation / emission 512,550nm/570,615nm.