MARCH5 Polyclonal Antibody, PE-Cy5.5 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-9339R-PE-Cy5.5-100uL |
| Product Name | MARCH5 Polyclonal Antibody, PE-Cy5.5 Conjugated |
| Applications |
WB
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | PE-Cy5.5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human MARCH5 |
| Immunogen Range | 1-100/278 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 54708 |
| Synonyms | E3 ubiquitin-protein ligase MARCH5; MARCH-V; Membrane-associated RING finger protein 5; Membrane-associated RING-CH protein V; RING finger protein 153. |
| Background | MARCH5 is a member of the E3 ubiquitin-protein ligase family. This mitochonrial E3 ubiquitin-protein ligase plays an importnat role in controlling the morphology of the mitochondria. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfer the ubiquitin to targeted substrates. MARCH5 has been shown to interact with MTFN2 and ubiquitinated forms of DRP1. |
| Application Dilution | |
| WB | 1:300-5000 |
Frequently asked questions
Which applications is MARCH5 Polyclonal Antibody, PE-Cy5.5 Conjugated (BS-9339R-PE-CY5-5) validated for, and at what dilution?
BS-9339R-PE-CY5-5 is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does MARCH5 Polyclonal Antibody, PE-Cy5.5 Conjugated (BS-9339R-PE-CY5-5) react with?
Tested reactivity: Human, Mouse, Rat.
How should BS-9339R-PE-CY5-5 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
What immunogen was used to raise MARCH5 Polyclonal Antibody, PE-Cy5.5 Conjugated (BS-9339R-PE-CY5-5)?
KLH conjugated synthetic peptide derived from human MARCH5 Immunogen range: 1-100/278.
What are the excitation and emission wavelengths of the PE-Cy5.5 conjugate (BS-9339R-PE-CY5-5)?
PE-Cy5.5 on BS-9339R-PE-CY5-5: excitation / emission 488nm/694nm.