MARCH7 Polyclonal Antibody, Biotin Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-9341R-Biotin-100uL |
| Product Name | MARCH7 Polyclonal Antibody, Biotin Conjugated |
| Applications |
WB
IHC-P
IP
|
| Reactivity | Human, Rat |
| Predicted Reactivity | Mouse, Dog, Cow, Sheep, Pig, Horse, Rabbit |
| Specifications | |
| Conjugation | Biotin |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human MARCH7 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C for 12 months. |
| Target | |
| Gene ID | 64844 |
| Swiss Prot | Q9H992 |
| Synonyms | AXO; AXOT; Axotrophin; E3 ubiquitin-protein ligase MARCH7; MARCH VII; MARCH-VII; March7; MARH7_HUMAN; Membrane associated RING CH protein VII; Membrane associated ring finger C3HC4 7 E3 ubiquitin protein ligase; membrane associated ring finger C3HC4 7; Membrane associated RING finger protein 7; Membrane-associated RING finger protein 7; Membrane-associated RING-CH protein VII; RING finger protein 177; RNF177. |
| Background | Axotrophin is a stem cell gene that encodes a protein which is involved in T lymphocyte regulation (especially in regulating the proliferation) and leukemia inhibitory factor (LIF) release. LIF is a neuropoietic cytokine that is important for stem cell regulation and thymocyte stimulation. Both Axotrophin and LIF are linked to transplantation intolerance. Axotrophin is also involved in corpus callosum differentiation and may play a role in glial cell line-derived neurotrophic factor (GDNF)-dependent sensory neuron survival in the substantia gelatinosa of the adult spinal cord. Axotrophin is primarily expressed in the hippocampus, cortex, purkinje and granule cells of the cerebellum. |
| Application Dilution | |
| WB | 1:300-5000 |
| IHC-P | 1:200-400 |
| IP | 1-2ug |
Frequently asked questions
Which applications is MARCH7 Polyclonal Antibody, Biotin Conjugated (bs-9341R-Biotin) validated for, and at what dilution?
bs-9341R-Biotin is validated for WB (1:300-5000); IHC-P (1:200-400); IP (1-2ug). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does MARCH7 Polyclonal Antibody, Biotin Conjugated (bs-9341R-Biotin) react with?
Tested reactivity: Human, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Dog, Cow, Sheep, Pig, Horse, Rabbit.
How should bs-9341R-Biotin be stored?
Store at -20C for 12 months. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise MARCH7 Polyclonal Antibody, Biotin Conjugated (bs-9341R-Biotin)?
KLH conjugated synthetic peptide derived from human MARCH7