MARCH8 Polyclonal Antibody, AbBy Fluor-405 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-9342R-BF405 |
| Product Name | MARCH8 Polyclonal Antibody, AbBy Fluor-405 Conjugated |
| Applications |
WB
IF(IHC-P)
|
| Reactivity | Rat |
| Predicted Reactivity | Human, Mouse, Cow, Sheep, Pig, Horse, Chicken, Rabbit |
| Specifications | |
| Conjugation | AbBy Fluor-405 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human MARCH8 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 220972 |
| Synonyms | c MIR; c-MIR; Cellular modulator of immune recognition; E3 ubiquitin protein ligase MARCH8; E3 ubiquitin-protein ligase MARCH8; MARCH 8; MARCH VIII; MARCH-VIII; MARCH8; MARH8_HUMAN; Membrane associated RING CH protein VIII; Membrane associated ring finger C3HC4 8; Membrane associated RING finger protein 8; Membrane-associated RING finger protein 8; Membrane-associated RING-CH protein VIII; MIR; RING finger protein 178; RNF178. |
| Background | The RING-type zinc finger motif is present in a number of viral and eukaryotic proteins and is made of a conserved cysteine-rich domain that is able to bind two zinc atoms. Proteins that contain this conserved domain are generally involved in the ubiquitination pathway of protein degradation. MARCH8 (membrane-associated ring finger (C3HC4) 8), also known as MIR or RNF178 (RING finger protein 178), is a 291 amino acid multi-pass membrane protein that localizes to vesicle membranes and contains one RING-CH-type zinc finger. Expressed in a variety of tissues, including immature dendritic cells, MARCH8 functions as an E3 ubiquitin ligase that is thought to regulate immune responses by promoting the ubiquitination and subsequent degradation of target proteins, such as B7-2 and CD71. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
Frequently asked questions
Which applications is MARCH8 Polyclonal Antibody, AbBy Fluor-405 Conjugated (bs-9342R-BF405) validated for, and at what dilution?
bs-9342R-BF405 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does MARCH8 Polyclonal Antibody, AbBy Fluor-405 Conjugated (bs-9342R-BF405) react with?
Tested reactivity: Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Mouse, Cow, Sheep, Pig, Horse, Chicken, Rabbit.
How should bs-9342R-BF405 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise MARCH8 Polyclonal Antibody, AbBy Fluor-405 Conjugated (bs-9342R-BF405)?
KLH conjugated synthetic peptide derived from human MARCH8
What are the excitation and emission wavelengths of the AbBy Fluor-405 conjugate (bs-9342R-BF405)?
AbBy Fluor-405 on bs-9342R-BF405: excitation / emission 401nm/421nm.