Proteasome 20S C2 Polyclonal Antibody, AbBy Fluor-750 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-9350R-BF750 |
| Product Name | Proteasome 20S C2 Polyclonal Antibody, AbBy Fluor-750 Conjugated |
| Applications |
WB
IF(IHC-P)
|
| Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Dog, Cow, Horse, Chicken |
| Specifications | |
| Conjugation | AbBy Fluor-750 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Proteasome 20S C2 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 5682 |
| Synonyms | 20S Proteasome 1; 30 kDa prosomal protein; HC 2; HC2; Macropain subunit C2; Macropain subunit nu; Multicatalytic endopeptidase complex subunit C2; NU antibody PROS 30; PROS-30; PROS30; Proteasome prosome macropain subunit alpha type 1; Proteasome alpha 1 subunit; Proteasome component C2; Proteasome nu chain; Proteasome subunit alpha type 1; Proteasome subunit alpha type I; Proteasome subunit alpha type-1; Proteasome subunit nu; Protein P30 33K; PSA1_HUMAN; PSC 2; PSC2; PSMA 1; psmA1. |
| Background | Ubiquitin-dependent proteolysis mediates selective destruction of various cell cycle regulators, transcription factors and tumor suppressors. In eukaryotic cells, selective breakdown of cellular proteins is ensured by their ubiquitination and subsequent degradation by the 26S proteasome. At specific stages of development, embryo- and tissue-specific components of the 26S proteasome form, facilitating proteolysis. 20S Proteasome ?, also designated macropain subunit C2 or PROS-30, is a prosomal protein involved in a non-lysosomal ATP/ubiquitin-dependent proteolytic pathway. The entire proteasome is composed of at least 15 non-identical subunits which form a highly-ordered ring-shaped structure. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
Frequently asked questions
Which applications is Proteasome 20S C2 Polyclonal Antibody, AbBy Fluor-750 Conjugated (bs-9350R-BF750) validated for, and at what dilution?
bs-9350R-BF750 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Proteasome 20S C2 Polyclonal Antibody, AbBy Fluor-750 Conjugated (bs-9350R-BF750) react with?
Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat, Dog, Cow, Horse, Chicken.
How should bs-9350R-BF750 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Proteasome 20S C2 Polyclonal Antibody, AbBy Fluor-750 Conjugated (bs-9350R-BF750)?
KLH conjugated synthetic peptide derived from human Proteasome 20S C2
What are the excitation and emission wavelengths of the AbBy Fluor-750 conjugate (bs-9350R-BF750)?
AbBy Fluor-750 on bs-9350R-BF750: excitation / emission 749nm/775nm.