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Proteasome 20S alpha 3 Antibody, AbBy Fluor-647 Conjugated

Applications

  • WB
  • IF(IHC-P)

Reactivity

  • Human
  • Rat

Predicted Reactivity

  • Mouse
  • Dog
  • Pig
  • Horse
  • Chicken
  • Rabbit
Overview
Catalog # bs-9352R-BF647
Product Name Proteasome 20S alpha 3 Antibody, AbBy Fluor-647 Conjugated
Applications
WB IF(IHC-P)
Reactivity Human, Rat
Predicted Reactivity Mouse, Dog, Pig, Horse, Chicken, Rabbit
Specifications
Conjugation AbBy Fluor-647
Host Rabbit
Source KLH conjugated synthetic peptide derived from human Proteasome 20S alpha 3
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 5684
Synonyms 20S Proteasome 3; HC8; Macropain subunit C8; Multicatalytic endopeptidase complex subunit C8; Proteasome prosome macropain subunit alpha type 3; Proteasome alpha 3 subunit; Proteasome component C8; Proteasome subunit alpha type 3; Proteasome subunit alpha type-3; Proteasome subunit C8; PSA3_HUMAN; PSC3; PSMA3.
Background The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. Binds to the C-terminus of CDKN1A and thereby mediates its degradation. Negatively regulates the membrane trafficking of the cell-surface thromboxane A2 receptor (TBXA2R) isoform 2.
Application Dilution
WB 1:300-5000
IF(IHC-P) 1:50-200

Frequently asked questions

Which applications is Proteasome 20S alpha 3 Antibody, AbBy Fluor-647 Conjugated (bs-9352R-BF647) validated for, and at what dilution?

bs-9352R-BF647 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does Proteasome 20S alpha 3 Antibody, AbBy Fluor-647 Conjugated (bs-9352R-BF647) react with?

Tested reactivity: Human, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Dog, Pig, Horse, Chicken, Rabbit.

How should bs-9352R-BF647 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise Proteasome 20S alpha 3 Antibody, AbBy Fluor-647 Conjugated (bs-9352R-BF647)?

KLH conjugated synthetic peptide derived from human Proteasome 20S alpha 3

What are the excitation and emission wavelengths of the AbBy Fluor-647 conjugate (bs-9352R-BF647)?

AbBy Fluor-647 on bs-9352R-BF647: excitation / emission 650nm/665nm.